Direct labeling of Octreotide with 99mTc and clinical study in patients with Graves’ ophthalmopathy

ZHAO Rong; WANG Jing; DENG Jing-lan; WANG Jiang

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Journal of China Clinic Medical Imaging ›› 2007, Vol. 18 ›› Issue (8) : 533-536.

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Direct labeling of Octreotide with 99mTc and clinical study in patients with Graves’ ophthalmopathy

ZHAO Rong, WANG Jing, DENG Jing-lan, WANG Jiang

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Abstract

Objective: Octreotide was labeled with 99mTc, to analyse radiochemical characters of 99mTc-Octreotide and investigate the clinical receptor imaging in patient with Graves’ ophthalmopathy(GO). Methods: Labeling was accomplished by reduction of the cysteine bridge, which provided sulfhydryl groups for chelation with 99mTc. The labeling rate was determined with Xinhua I chromatography. The radiochemical property of　99mTc-OCT were evaluated by Sep pak Cl8 chromatography, the cysteine challenge experiment, the serum incubation test and the stability experiment in vitro and the clinical procedures of receptor fusion imaging were carried out in patient with GO. Results: The labeling rate of 99mTc-OCT was (95.8±0.55)％, the99mTc-OCT compound has excellent radiochemical characters. SRS showed markedly increased orbital uptake of 99mTc-OCT in patient with active GO. Conclusion: 99mTc-OCT can be readily prepared, the labeling yields are quite high(>95％), and stability in vivo(vitro); 99mTc-OCT is a promising radiopharmaceutical for somatostatin receptor scintigraphic imaging.

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hyperthyroidism / radionuclide imaging

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ZHAO Rong;WANG Jing;DENG Jing-lan;WANG Jiang. Direct labeling of Octreotide with 99mTc and clinical study in patients with Graves’ ophthalmopathy[J]. Journal of China Clinic Medical Imaging. 2007, 18(8): 533-536