Effect of the vascular endothelial growth factor and tumor growth after treatment withinterferon α1b-lipiodol emulsion in a rabbit model of liver cancer

SI Guang-yan; ZHOU Shi; WANG Xue-jian; ZHANG Wei; GUO Jian-xing; JIANG Tian-peng

Journal of China Clinic Medical Imaging ›› 2008, Vol. 19 ›› Issue (1) : 30-34.

论著

Effect of the vascular endothelial growth factor and tumor growth after treatment withinterferon α1b-lipiodol emulsion in a rabbit model of liver cancer

SI Guang-yan1, ZHOU Shi2, WANG Xue-jian2, ZHANG Wei2, GUO Jian-xing2, JIANG Tian-peng2

Author information +

History +

Abstract

Objective: To investigate effect of vascular endothelial growth factor(VEGF) and tumor growth after transarterial chemoembolization with interferon α1b-lipiodol emulsion in a rabbit model bearing VX2 liver tumor. Methods: To establish tumor model in the left anterior lobe of the liver of the Japanese white rabbits via laparotomic route. Thirty rabbits were successfully implanted and were hypervascular detected by color Doppler ultrasound after 2 weeks. Then were divided into 3 groups randomly, 10 in each group. In group I, only angiography was performed after percutaneous trans-femoral retrograde approach of hepatic arterial catheterization. In group II, transcatheter arterial embolization with 0.6ml super liquid iodinated oil were carried out after hepatic angiography. In group III, 0.6ml super liquid iodinated oil and interferon α1b 7.5×106U emulsion were given to each rabbit instead of super liquid iodinated oil. To observe the period from resuscitation of anesthesia to normal eating and common condition after surgery. All the rabbits were killed 14 days postoperatively. The tumor sizes were measured, the numbers of metastasis in liver were calculated. Condition of metastasis in abdominal cavity was observed. The expression of VEGF in the specimen that was obtained in tumor liver tissue and surrounding normal liver tissue(4 groups per-rabbit) was examined by immunohistochemistry. Results: Differences of the mean period from resuscitation of anesthesia to normal eating among the group II and III were not significant(P>0.05), but significantly more prolongation than group I(P<0.05). Other common condition was not significant. Differences of the sizes of primary tumor among the group II and III were not significant(P>0.05), but significantly smaller than group I(P<0.05). The number of metastasis in group II was more than that of group I and III(P<0.05); in group I and III the group I and II the number of metastasis were not significant(P>0.05). The expression of VEGF in the tumor and normal liver tissue were not significant in group I and III(P>0.05). In group II the expression of VEGF was significantly higher than that in group I and III(P<0.05). Conclusion: Transcatheter arterial chemoembolization(TAE) probably promotes the expression of VEGF and metastasis in liver. TAE with interferon α1b-lipiodol emulsion could inhibit the expression of VEGF after merely TAE, inhibit tumorous growth and prevent metastasis in liver.

Key words

Liver neoplasms / experimental / Rabbits / Vascular endothelial growth factor A / Interferon-alpha / Embolization / therapeutic

Cite this article

EndNote

Ris (Procite)

Bibtex

Download Citations

SI Guang-yan;ZHOU Shi;WANG Xue-jian;ZHANG Wei;GUO Jian-xing;JIANG Tian-peng. Effect of the vascular endothelial growth factor and tumor growth after treatment withinterferon α1b-lipiodol emulsion in a rabbit model of liver cancer[J]. Journal of China Clinic Medical Imaging. 2008, 19(1): 30-34