Objective: To study the diagnostic value of CT virtual endoscopy(CTVE) and relative post-processing techniques using 64 multi-slice spiral CT(MSCT) for intestinal diseases. Methods: Seventy-five cases with clinically suspected intestinal diseases had MSCT enhancement scanning with air filling of the bowel lumen. Image post-processing were done at the ADW 4.4 workstation, including CTVE, MPR, VR, VD and MIP images. Comparison between the diagnostic of MSCT and the results of surgery, pathology and clinical follow-up was performed to assess the diagnosis value of 64 MSCT for intestinal diseases. Results: Of the 75 cases examined, 49 cases had organic diseases by combination diagnosis. MSCT identified positive findings in 45 patients. Five cases were missed and 1 was false positive. The sensitivity, specificity, positive predictive value, negative predictive value, and accuracy was 89.9%, 96.2%, 97.8%, 83.3% and 92% respectively for MSCT. The subtle lesions of the mucosal surface and morphological changes were well showed by CTVE. Combined with other post-processing methods, intestinal diseases could be diagnosed correctly. Conclusion: CTVE and relative post-processing techniques of 64 MSCT are of significant clinical value in diagnosing intestinal diseases.