The effect of different doses of hypotonic contrast agent on the dog tubular epithelial cell apoptosis andthe expression of Caspase-3

RAN Wei; WANG Zhi-ming; QIAN Ying; XU Na; ZHENG Shi-lei

Journal of China Clinic Medical Imaging ›› 2012, Vol. 23 ›› Issue (5) : 327-330.

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The effect of different doses of hypotonic contrast agent on the dog tubular epithelial cell apoptosis andthe expression of Caspase-3

RAN Wei1, WANG Zhi-ming2, QIAN Ying1, XU Na1, ZHENG Shi-lei1

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Abstract

Objective: To study the effect of hypotonic ion-contrast agents dose on the apoptosis of canine renal tubular epithelial cell and the expression of Caspase-3, so that to provide the evidence that low dose of hypotonic contrast agents can help reduce the side effects. Materials and Methods: Different doses(1.5 mL/kg, 1.0 mL/kg) of Iopromiden(300 mgI/mL) were injected with high pressure(1.0 mL/s) for dogs in study groups. Control group received an equal dose injection of normal saline. After 48 hours, kidneys were taken out. TUNEL dyeing was done to detect tubular epithelial cell apoptosis, and S-P stain was to observe Caspase-3 expression. Western blot was used to semi-quantitatively observe the Caspase-3 expression in kidney medulla. Results: After injection of contrast agents, tubular epithelial cell apoptosis rate and Caspase-3 expression significantly increased than control group(P<0.01). And the high dose group increased more obviously than the low dose group（P<0.01）. Conclusion: Regular doses of hypotonic iodine-contrast agents commonly used in clinics can also cause apoptosis of the dog kidney tubular epithelial cell, which is relative to the dosage. Low dose contrast agents can reduce side effects. Caspase-3 may be involved in the tubular epithelial cell apoptosis controlled by the contrast agents.

Key words

Epithelial cells / Kidney tubules / Apoptosis / Contrast media

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RAN Wei;WANG Zhi-ming;QIAN Ying;XU Na;ZHENG Shi-lei. The effect of different doses of hypotonic contrast agent on the dog tubular epithelial cell apoptosis andthe expression of Caspase-3[J]. Journal of China Clinic Medical Imaging. 2012, 23(5): 327-330