Comparative studies of the 18F-FDG cell binding the drug-resistant breast cancer cell Bcap37/MDR1 and breast cancer cell Bcap37

XIAO Han; ZHANG Wei; ZHANG Bin; DENG Sheng-ming; ZHOU Hai-zhong

Journal of China Clinic Medical Imaging ›› 2013, Vol. 24 ›› Issue (8) : 551-554.

论著

Comparative studies of the 18F-FDG cell binding the drug-resistant breast cancer cell Bcap37/MDR1 and breast cancer cell Bcap37

XIAO Han1, ZHANG Wei2, ZHANG Bin2, DENG Sheng-ming2, ZHOU Hai-zhong1

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Abstract

Objective: To compare the 18F-FDG cell binding in the drug-resistan breast cancer cell Bcap37/MDR1 and breast cancer cell Bcap37, to lay the foundation for a further study of the inhibitory effect of chemotherapy in breast cancer cell Bcap37/MDR1 and breast cancer cell Bcap37. Methods: Several different binding conditions were tested. ①The cell number was 1.25×105~1×107/bottle. ②The reaction time was 20~120 min. ③The radioactivity of 18F-FDG was 1.85~29.6 KBq. ④The glucose concentration was 0~5.5 mmol/L. The count of CPM(B) in cells and CPM(F) in supernatant were measured by the γ equipment. The binding efficiency of Bcap37 and Bcap37/MDR1 cell with 18F-FDG were calculated and compared. Result: When other conditions were invariable, and separately changed cell number, the reaction time and the 18F-FDG radioactivity, the binding efficiency of Bcap37 was higher than the Bcap37/MDR1. The difference was significant(P<0.05). The difference of binding efficiency between Bcap37 and Bcap37/MDR1 cell at the 0 and 1.39 mmol/L groups was significant(P<0.05); The difference of binding efficiency between Bcap37 and Bcap37/MDR1 cell at 2.78 mmol/L and 5.5 mmol/L groups was not statistically significant(P>0.05). Conclusions: The 18F-FDG cell binding of breast cancer Bcap37/MDR1 was lower than the Bcap37. It showed that the proliferation and metabolism of drug resistance of tumor cells were lower than the parental tumor cells.

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Breast neoplasms / Fluorodeoxyglucose F18 / Radionuclide imaging

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XIAO Han;ZHANG Wei;ZHANG Bin;DENG Sheng-ming;ZHOU Hai-zhong. Comparative studies of the 18F-FDG cell binding the drug-resistant breast cancer cell Bcap37/MDR1 and breast cancer cell Bcap37[J]. Journal of China Clinic Medical Imaging. 2013, 24(8): 551-554