Sonographic findings of the neovascularization in the retained liver VX2 tumor ofrabbit after radiofrequency ablation: an experimental study

WANG Jian-lin; LIU Ying; FAN Qiu-yang; MA Yan-yan; ZHANG Jing-zhuo; QIU Tian-shuang

Journal of China Clinic Medical Imaging ›› 2013, Vol. 24 ›› Issue (9) : 639-643.

论著

Sonographic findings of the neovascularization in the retained liver VX2 tumor ofrabbit after radiofrequency ablation: an experimental study

WANG Jian-lin1, LIU Ying1, FAN Qiu-yang1, MA Yan-yan1, ZHANG Jing-zhuo1, QIU Tian-shuang2

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Abstract

Objective: To observe the sonographic findings of the residual tumor in different time intervals after radiofrequency ablation(RFA) treatment to rabbit VX2 liver tumor, and to study the changes of vascular endothelial grow factor(VEGF) and the expression of microvascular density(MVD) in the residual tumor tissue. Method: The rabbit VX2 liver cancer model was established, and radius of the radiofrequency was controlled under ultrasound guidance, then the residual VX2 liver tumor in rabbit was established. The specimens before and after treatment at 0 h, 1 w, 2 w, 4 w were acquired for ultrasonic observation. It was to observe the expression of VEGF and MVD in the residual cancer tissue by the immune histochemical method. Results: ①Both the 1 w and 2 w residual tumor were smaller and the vascularization were lesser than those of the 4 w specimen. The signal of vascularization reappeared at 4 w specimen. ②Compared with the tumor before RFA, the expression of VEGF and MVD in the residual tumor tissues at 0 h, 1 w, 2 w decreased(P<0.05). However compared with the tumor before RFA and the residual tumor tissues at 0 h, 1 w, 2 w, the expression of VEGF and MVD in 4 w specimen increased(P<0.05). Conclusion: In the short term(within 2 w) RFA destroys tumor vessels thus controls and inhibits VEGF to obtain short term curative effect, but in the long term can induce neovascularization due to hypoxia and tumor recurrency occurred.

Key words

Liver neoplasms / Neoplasm / residual / Ultrasonography / Doppler / color / Rabbits

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WANG Jian-lin;LIU Ying;FAN Qiu-yang;MA Yan-yan;ZHANG Jing-zhuo;QIU Tian-shuang. Sonographic findings of the neovascularization in the retained liver VX2 tumor ofrabbit after radiofrequency ablation: an experimental study[J]. Journal of China Clinic Medical Imaging. 2013, 24(9): 639-643