Research on correlation between aquaporin magnetic resonance molecular imaging and AQP4 expression

CHEN Qiu-yan; WU Fu-lin; PENG Xiao-lan; LI Chun-li; JIANG Min; CHEN Ting-ting; WEI Ding-tai

Journal of China Clinic Medical Imaging ›› 2016, Vol. 27 ›› Issue (12) : 837-841.

CHEN Qiu-yan, WU Fu-lin, PENG Xiao-lan, LI Chun-li, JIANG Min, CHEN Ting-ting, WEI Ding-tai

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Abstract

Objective: To explore the correlation between aquaporin magnetic resonance molecular imaging(AQP MRI) and the expression of AQP4. Methods: Ten adult SD male rats were subjected to either predisposing(LRP) or sham surgery and then subsequently underwent right middle cerebral artery occlusion(MCAo) for one hour. AQP MRI was executed 48 h after reperfusion. And the AQP4 expression was detected by Western blot. Results: The success rate of modeling was 60%, meaning there were only 6 rats living up to the criteria of modeling. AQP MRI showed AQP ADC values of the affected side of rats No.1~5 were lower than those of the uninjured side, but the AQP ADC values in the affected side of rat No.6 was much higher than that of the uninjured side. Western blot results showed that the AQP4 expression in the affected side of No.1~6 rats was higher than that of the uninjured side. Correlation analysis showed that the AQP ADC values were negatively correlated to the level of AQP4 protein expression when excluding rat No.6. Conclusion: AQP MRI can display the expression quantity as well as the distribution of AQP4 in vivo, and it has great value in clinical application.

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Brain ischemia / Magnetic resonance imaging

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CHEN Qiu-yan, WU Fu-lin, PENG Xiao-lan, LI Chun-li, JIANG Min, CHEN Ting-ting, WEI Ding-tai. Research on correlation between aquaporin magnetic resonance molecular imaging and AQP4 expression[J]. Journal of China Clinic Medical Imaging. 2016, 27(12): 837-841

References

[1]白岩，赵周社，史大鹏，等. 水通道蛋白成像技术及其在脑胶质瘤中的应用进展[J]. 功能与分子医学影像学：电子版，2015，4（2）：672-676.
[2]李佳慧，李秋菊，于兵，等． DWI-MRI多ｂ值水通道蛋白分子成像机理和方法学研究[Ｊ]．中国临床医学影像杂志，2014，25（3）：186-189.
[3]李秋菊，李佳慧，赵周社，等． DWI多b值水通道蛋白分子成像在肝脏纤维化早期诊断的价值[Ｊ]．中国临床医学影像杂志，2014，25（10）：719-723.
[4]陈秋雁，吴富淋，缪绍维，等. 肢体远端缺血预处理延缓脑卒中DWI上病灶出现的机制研究[J]. 中风与神经疾病杂志，2014，31（3）：247-250.
[5]魏鼎泰，陈秋雁，吴富淋，等. 肢体远端缺血预处理对抑制缺血性脑卒中的MR DWI研究[J]. 中风与神经疾病杂志，2012，29（8）：691-694.
[6]Ren C, Gao X, GK S, et al. Limb remote-preconditioning protects against focal ischemia in rats and contradicts the dogma of therapeutic time windows for preconditioning[J]. Neuroscience, 2008, 151(4): 1099-1103.
[7]林艳红，孙夕林，程雁，等. 水通道蛋白分子成像研究[J]. 放射学实践，2015，30（6）：622-625.　
[8]Agre P, Sasaki S, Chrispeels MJ． Aquaporins a family of water channel proteins Ⅲ[J]． Am J Physiol Renal Physial, 1993, 3(265): F461．
[9]Benga G． Birth of water channel proteins-the aquaporins[J]． Cell Bio Int, 2003, 9(27): 701-709．
[10]Zelenina M． Regulation of brain aquaporins[J]． Neurochem Int, 2010, 57(4): 468-488．
[11]Ishibashi K, Hara S, Kondo S． Aquaporin water channels in mammals[J]． Clin Exp Nephrol, 2009, 13(2): 107-117．
[12]Badaut J, Fukuda AM, Jullienne A, et al. Aquaporin and brain diseases[J]. Biochim Biophys Acta, 2014, 1840(5): 1554-1565.
[13]Brown PD, Davies SL, Speake T, et al. Molecular mechanisms of cerebrospinal fluid production[J]. Neuroscience, 2004, 129(4): 955-968.
[14]Shields SD, Mazario J, Skinner K, et al. Anatomical and functional analysis of aquaporin 1, a water channel in primary afferent neurons[J]. Pain, 2007, 131(1-2): 8-20.
[15]Badaut J, Hirt L, Granziera C, et al. Astrocyte-specific expression of aquaporin-9 in mouse brain is increased after transient focal cerebral ischemia[J]. J Cereb Blood Flow Metab, 2001, 21(5): 477-482.
[16]Badaut J, Petit JM, Brunet JF, et al. Distribution of aquaporin 9 in the adult rat brain: preferential expression in catecholaminergic neurons and in glial cells[J]. Neuroscience, 2004, 128(1): 27-38.
[17]de Castro Ribeiro M, Hirt L, Bogousslavsky J, et al. Time course of aquaporin expression after transient focal cerebral ischemia in mice[J]. J Neurosci Res, 2006, 83(7): 1231-1240.
[18]Yasui M． Aquaporin from basic to clinical medicine: roles in brain edema[J]． No To Hattatsu, 2011, 43(3): 191-194．
[19]Lu H, Sun SQ． A correlative study between AQP4 expression and the manifestation of DWI after the acute ischemic brain edema in rats[J]． Chin Med J(Engl), 2003, 116(7): 1063－1069．
[20]Kleindienst A, Fazzina G, Amorini AM, et al． Modulation of AQP4 expression by the protein kinase C activator, phorbol myristate acetate, decreases ischemia-induced brain edema[J]． Acta Neurochir Suppl, 2006, 96: 393－397．
[21]Cai SN, Zhu SM． The effects of ketamine pretreated on cerebral edema and AQP4 expression after transient focal cerebral ischemia/reperfusion in rats[J]． Zhonghua Yi Xue Za Zhi, 2010, 9(23): 1648－1651．
[22]Qi LL, Fang SH, Shi WZ, et al． CysLT2 receptor-mediated AQP4 upregulation is involved in ischemic-like injury through activation of EＲK and p38 MAPK in rat astrocytes[J]. Life Sci, 2011, 88(1-2): 50-56．
[23]Badaut J, Ashwal S, Obenans A． Aquaporins in cerebrovascular disease: a target for treatment of brain edema?[J]. Cerebrovas Dis, 2011, 31(6): 521-531．
[24]Steiner E, Enzmann GU, Lin S, et al. Loss of astrocyte polarization upon transient focal brain ischemia as a possible mechanism to counteract early edema formation[J]. Glia, 2012, 60(11): 1646-1659.
[25]Amiry-Moghaddam M, Otsuka T, Hurn PD, et al. An alpha-syntrophin-dependent pool of AQP4 in astroglial end-feet confers bidirectional water flow between blood and brain[J]. Proc Natl Acad Sci USA, 2003, 100(4): 2106-2111.
[26]Kiening KL, Van Landeghem FK, Schreiber S, et al. Decreased hemispheric Aquaporin-4 is linked to evolving brain edema following controlled cortical impact injury in rats[J]. Neurosci Lett, 2002, 324(2): 105-108.
[27]Zeng XN, Xie LL, Liang R, et al. AQP4 knockout aggravates ischemia/reperfusion injury in mice[J]. CNS Neurosci Ther, 2012, 18(5): 388-394.
[28]Papadopoulos MC, Manley GT, Krishna S, et al. Aquaporin-4 facilitates reabsorption of excess fluid in vasogenic brain edema[J]. FASEB J, 2004, 18(11): 1291-1293.
[29]Ribeiro Mde C, Hirt L, Bogousslavsky J, et al. Time course of aquaporin expression after transient focal cerebral ischemia in mice[J]. J Neurosci Res, 2006, 83(7): 1231-1240.
[30]Vella J, Zammit C, Di Giovanni G, et al. The central role of aquaporins in the pathophysiology of ischemic stroke[J]. Front Cell Neurosci, 2015, 9: 108.
[31]He Z, Wang X, Wu Y, et al. Treadmill pre-training ameliorates brain edema in ischemic stroke via down-regulation of aquaporin-4: an MRI study in rats[J]. PLoS One, 2014, 9(1): e84602.