Study on the correlation between ultra-high-b-value diffusion-weighted MR imaging and #br#
aquaporin-4 in a hepatic ischemia-reperfusion model in rats

ZHANG Si-ying1; YAO Lin-peng1; WANG Qi-dong1; HONG Yuan2; XUE Xing1; KONG De-xing2; CHEN Feng1

Journal of China Clinic Medical Imaging ›› 2017, Vol. 28 ›› Issue (10) : 731-735.

Study on the correlation between ultra-high-b-value diffusion-weighted MR imaging and #br# aquaporin-4 in a hepatic ischemia-reperfusion model in rats

ZHANG Si-ying1, YAO Lin-peng1, WANG Qi-dong1, HONG Yuan2, XUE Xing1, KONG De-xing2, CHEN Feng1

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Abstract

Objective: To study the expression of aquaporin-4(AQP-4) in hepatic ischemia-reperfusion tissue, and to explore the correlation between ultra-high-b-value magnetic resonance diffusion-weighted imaging(DWI) and the expression of AQP-4. Methods: Forty-two SD rats weighting 250～300 g were divided into 7 groups(n=6). The rats in the experimental group were treated with ligating the right lobe portal vein and hepatic artery for 3 h. The rats in the control group were treated with sham operation. 3.0T MRI scanning including plain scan T1WI, T2WI, conventional DWI(b=0 s/mm2, 1 000 s/mm2) and 15 b-values DWI(b=0～4 500 s/mm2) were performed in both groups at 0 h, 6 h, 12 h, 1 d, 3 d, 7 d and 14 d after reperfusion. Standard apparent diffusion coefficient(ADCs) and ultra-high apparent diffusion coefficient(ADCu) were measured based on single b-value DWI and 15 b-values DWI respectively. The dynamic changes of each parameter were observed at each time point. After scanning, the animals were euthanized and liver specimens were taken for HE staining. The morphological changes of the liver were noted under light microscope. The expression of AQP-4 was measured qualitatively and quantitatively by Western blot. Results: The ADCs in the experimental group were higher than those of the control group starting from 12 hours after operation and returned to normal at 7 d after operation. ADCu in the experimental group began to significantly higher than those in the control group at 6 h after operation and returned to normal at 14 d after operation. The expression of AQP-4 in the experimental group decreased gradually at 6 h, 12 h, 1 d and 3 d after operation but still higher than that in the control group and began to approach normal at 7 d after operation. ADCs did not correlate with AQP-4 expression(P=0.416), but ADCu was significantly correlated with AQP-4 expression(P=0.034) and the correlation coefficient was 0.721. Conclusion: The ultra-high-b-value DWI can reflect the transport of AQPs.

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Liver / Reperfusion injury / Rats / Magnetic resonance imaging

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ZHANG Si-ying1, YAO Lin-peng1, WANG Qi-dong1, HONG Yuan2, XUE Xing1, KONG De-xing2, CHEN Feng1. Study on the correlation between ultra-high-b-value diffusion-weighted MR imaging and #br# aquaporin-4 in a hepatic ischemia-reperfusion model in rats[J]. Journal of China Clinic Medical Imaging. 2017, 28(10): 731-735

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