摘要
目的:探讨血管内皮细胞生长因子(VEGF)对家兔局灶性脑缺血再灌注损伤的治疗作用及MR影像学评价。方法:采用兔大脑中动脉阻断(MCAO)局灶性脑缺血再灌注模型,在缺血2h再灌注损伤后即刻应用微量进样器将不同剂量的VEGF立体定向导入梗死灶周,于再灌注72h,观察梗死体积、灶周缺血半暗带caspase-3蛋白表达、凋亡率、ADC值比率(ADCR),评价神经功能缺损。结果:缺血再灌注即刻灶周给予VEGF,1.25ng/μl VEGF的应用对脑水肿、神经功能恢复和血管形成影响不大,而2.5ng/μl和5.0ng/μl的VEGF应用明显降低脑含水量、灶周皮层凋亡率及caspase-3活性表达,ADCR升高,脑组织表面血管增粗、增加,2.5ng/μl组的变化较5.0ng/μl组更明显,同时也能明显降低梗死体积(P<0.05)。结论:2.5ng/μl VEGF应用治疗家兔局灶性脑缺血再灌注损伤可诱导血管发生和神经保护作用,是进一步研究和评价VEGF治疗效应的最佳剂量。
Abstract
Objective: To study the dose-effectiveness of the vascular endothelial growth factor(VEGF) in focal cerebral ischemia reperfusion injury of rabbits by MR imaging. Methods: Rabbit model of focal cerebral ischemia was induced by occlusion of the middle cerebral artery for 2 hours. The VEGF was administered immediately after reperfusion at different doses. We measured infarct volume, neural cell apoptosis, activation of caspase-3 and ADCR in the perifocal regions of cerebral ischemia and neurological deficit score 72h after reperfusion. Results: There was no significant difference in the cerebral edema, neuroprotective efficacy and angiogenesis at the dose of 1.25ng/μl VEGF compared with control group. When administered VEGF at the dose of 2.5ng/μl and 5.0ng/μl after reperfusion, the cerebral edema, the percentage of apoptotic cell and activation of caspase-3 in the perifocal regions were lowered significantly, the ADCR and increase of blood vessels were significantly higher than those in the control group. The neuroprotective efficacy and significant enlargement of the blood vessels were observed in the 2.5ng/μl than that in 5.0ng/μl group, and the infarct volume was significantly reduced in the 2.5ng/μl group(P<0.05). Conclusions: The VEGF at a dose of 2.5ng/μl has displayed excellent increase in vessel density and neuroprotective efficacy for focal cerebral ischemia reperfusion injury of rabbits. Therefore, the dose of 2.5ng/μl was used in the following treatments to further evaluate the effect of VEGF.
关键词
再灌注损伤 /
血管内皮生长因子类 /
磁共振成像
Key words
reperfusion injury /
vascular endothelial growth factors /
magnetic resonance imaging
刘怀军;杨冀萍;刘瑞春;王志红;王藏海;池 琛;崔彩霞.
VEGF对兔局灶性脑缺血再灌注损伤治疗作用的剂量-效应关系及MR影像学评价[J]. 中国临床医学影像杂志. 2006, 17(9): 499-504
LIU Huai-jun;YANG Ji-ping;LIU Rui-chun;WANG Zhi-hong;WANG Cang-hai;CHI Chen;CUI Cai-xia.
The dose-effectiveness of VEGF in focal cerebral ischemia reperfusion injury of rabbits evaluated by MR imaging[J]. Journal of China Clinic Medical Imaging. 2006, 17(9): 499-504
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