干扰素α1b-碘化油乳剂对家兔移植肝癌模型VEGF及肿瘤生长的影响

斯光晏; 周石; 王学建; 张巍; 郭剑星; 蒋天鹏

中国临床医学影像杂志 ›› 2008, Vol. 19 ›› Issue (1) : 30-34.

论著

干扰素α1b-碘化油乳剂对家兔移植肝癌模型VEGF及肿瘤生长的影响

斯光晏1，周石2，王学建2，张巍2，郭剑星2，蒋天鹏2

作者信息 +

Effect of the vascular endothelial growth factor and tumor growth after treatment withinterferon α1b-lipiodol emulsion in a rabbit model of liver cancer

SI Guang-yan1, ZHOU Shi2, WANG Xue-jian2, ZHANG Wei2, GUO Jian-xing2, JIANG Tian-peng2

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摘要

目的：探讨经肝动脉使用干扰素α1b-碘化油乳剂对家兔移植VX2肝癌血管内皮生长因子（VEGF）及肿瘤生长的影响。方法：通过开腹接种法在日本大白兔左前叶肝脏建立荷瘤兔模型，2周后经彩色多普勒超声检查肝脏有肿瘤生长无腹水且血供丰富的兔作为研究对象。I组（n=10）：仅行经股动脉肝动脉插管；II组（n=10）：行经肝动脉超液态碘油栓塞；III组（n=10）：行经肝动脉干扰素α1b-碘化油乳剂栓塞。观察家兔的麻醉苏醒至正常进食时间及其一般情况。14天后处死家兔，测量原发肿瘤的大小、计数肝内转移灶的数目、腹腔有无转移、免疫组织化学染色检测VEGF的表达。结果：家兔麻醉苏醒至正常进食时间II、III组间无明显差异（P>0.05），但较I组明显延长（P<0.05）；其它一般情况无明显差异；肝内平均原发灶大小II、III组间无明显差异（P>0.05），较I组明显缩小（P<0.05）；肝内平均转移灶数目II组比III组明显增多（P<0.05），I、III组间及I、II组间无明显差异（P>0.05）；肿瘤内及正常肝组织内测VEGF I、III两组之间无明显差异（P>0.05），II组VEGF的阳性率明显高于第I、III组（P<0.05）。结论：经肝动脉栓塞肝癌可能促进VEGF的表达而导致肝内转移，干扰素α1b-碘化油乳剂行肝动脉栓塞可抑制单纯栓塞治疗后VEGF的增强表达，控制原发灶生长和肝内转移。

Abstract

Objective: To investigate effect of vascular endothelial growth factor(VEGF) and tumor growth after transarterial chemoembolization with interferon α1b-lipiodol emulsion in a rabbit model bearing VX2 liver tumor. Methods: To establish tumor model in the left anterior lobe of the liver of the Japanese white rabbits via laparotomic route. Thirty rabbits were successfully implanted and were hypervascular detected by color Doppler ultrasound after 2 weeks. Then were divided into 3 groups randomly, 10 in each group. In group I, only angiography was performed after percutaneous trans-femoral retrograde approach of hepatic arterial catheterization. In group II, transcatheter arterial embolization with 0.6ml super liquid iodinated oil were carried out after hepatic angiography. In group III, 0.6ml super liquid iodinated oil and interferon α1b 7.5×106U emulsion were given to each rabbit instead of super liquid iodinated oil. To observe the period from resuscitation of anesthesia to normal eating and common condition after surgery. All the rabbits were killed 14 days postoperatively. The tumor sizes were measured, the numbers of metastasis in liver were calculated. Condition of metastasis in abdominal cavity was observed. The expression of VEGF in the specimen that was obtained in tumor liver tissue and surrounding normal liver tissue(4 groups per-rabbit) was examined by immunohistochemistry. Results: Differences of the mean period from resuscitation of anesthesia to normal eating among the group II and III were not significant(P>0.05), but significantly more prolongation than group I(P<0.05). Other common condition was not significant. Differences of the sizes of primary tumor among the group II and III were not significant(P>0.05), but significantly smaller than group I(P<0.05). The number of metastasis in group II was more than that of group I and III(P<0.05); in group I and III the group I and II the number of metastasis were not significant(P>0.05). The expression of VEGF in the tumor and normal liver tissue were not significant in group I and III(P>0.05). In group II the expression of VEGF was significantly higher than that in group I and III(P<0.05). Conclusion: Transcatheter arterial chemoembolization(TAE) probably promotes the expression of VEGF and metastasis in liver. TAE with interferon α1b-lipiodol emulsion could inhibit the expression of VEGF after merely TAE, inhibit tumorous growth and prevent metastasis in liver.

导出引用

斯光晏;周石;王学建;张巍;郭剑星;蒋天鹏. 干扰素α1b-碘化油乳剂对家兔移植肝癌模型VEGF及肿瘤生长的影响[J]. 中国临床医学影像杂志. 2008, 19(1): 30-34

SI Guang-yan;ZHOU Shi;WANG Xue-jian;ZHANG Wei;GUO Jian-xing;JIANG Tian-peng. Effect of the vascular endothelial growth factor and tumor growth after treatment withinterferon α1b-lipiodol emulsion in a rabbit model of liver cancer[J]. Journal of China Clinic Medical Imaging. 2008, 19(1): 30-34

中图分类号： Ｒ735.7 Ｒ815