RGD序列多肽与不同肿瘤细胞结合特性研究

贾莹莹; 钱海利; 唐萌; 赵周社; 李宏利; 辛军; 张立生; 林晨

中国临床医学影像杂志 ›› 2008, Vol. 19 ›› Issue (1) : 35-37.

论著

RGD序列多肽与不同肿瘤细胞结合特性研究

贾莹莹1，钱海利2，唐萌1，赵周社3，李宏利3，辛军4，张立生5，林晨2

作者信息 +

Comparison of combination specification of the different tumor cell with RGD peptide

JIA Ying-ying1, QIAN Hai-li2, TANG Meng1, ZHAO Zhou-she3, LI Hong-li3, XIN Jun4, ZHANG Li-sheng5, LIN Chen2

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文章历史 +

摘要

目的：通过体外细胞和动物体内研究期望获得不同肿瘤细胞整合素αvβ3受体表达的资料，以指导临床研究。材料和方法：采用荧光显微镜检测FITC标记HYNIC-RGD对人胰腺癌、肝癌、肠癌和子宫颈癌细胞的结合；用99mTc-HYNIC-RGD在小鼠乳腺和肝癌动物模型上比较了肿瘤对99mTc-HYNIC-RGD的摄取。结果：体外研究表明胰腺癌和肝癌细胞对RGD结合明显高于肠癌和子宫颈癌。注射后3小时小鼠皮下接种的乳腺癌和肝癌肿瘤组织对99mTc-HYNIC-RGD的摄取达到高峰，乳腺癌对99mTc-HYNIC-RGD摄取高于肝癌40%以上。结论：体外研究表明胰腺癌和肝癌细胞均对RGD有高的摄取，活体小鼠动物模型研究表明乳腺癌对RGD具有高的摄取。

Abstract

Objective: To get the αvβ3 receptor combination information of the different tumor cells with RGD peptide by vivo and vitro method, so as to guide clinical molecular imaging research. Materials and Methods: Comparison combination specification of the pancreas tumor cell, hepatic tumor cell, intestine tumor cell and cervix tumor cell with FITC-HYNIC-RGD, and acquisition data for the xenograft mice model of breast tumor and hepatic tumor cell after IV injection of 99mTc-HYNIC-RGD. Results: Combination number of the pancreas tumor cell, hepatic tumor cell is higher than intestine tumor cell and cervix tumor cell with FITC-HYNIC-RGD. The uptake of breast tumor cell with 99mTc-HYNIC-RGD is 40% higher than that of hepatic tumor cell after injection. Conclusion: Combination of the pancreas tumor cell, hepatic tumor cell with FITC-HYNIC-RGD is the highest. Uptake of breast tumor with xenograft mice model is higher than hepatic tumor after injection of 99mTc-HYNIC-RGD.

导出引用

贾莹莹;钱海利;唐萌;赵周社;李宏利;辛军;张立生;林晨. RGD序列多肽与不同肿瘤细胞结合特性研究[J]. 中国临床医学影像杂志. 2008, 19(1): 35-37

JIA Ying-ying;QIAN Hai-li;TANG Meng;ZHAO Zhou-she;LI Hong-li;XIN Jun;ZHANG Li-sheng;LIN Chen. Comparison of combination specification of the different tumor cell with RGD peptide[J]. Journal of China Clinic Medical Imaging. 2008, 19(1): 35-37

中图分类号： Ｒ730.44 Ｒ817.4