摘要
目的：运用CT灌注成像评价大鼠C6脑胶质瘤生长过程中血流量（CBV）和血管通透性表面值（PS）的变化及其与VEGF表达的相关性。方法：采用立体定向方法在30只雌性Wistar大鼠右侧尾状核接种C6细胞，于接种后1周、2周、3周分别进行CT灌注成像，每次检查结束后留取鼠脑标本作病理学检查。结果：各组肿瘤区CBV和PS值均比对侧正常脑组织PS值增大（P<0.05），荷瘤2周及3周组肿瘤中心区PS值比相应肿瘤周边区PS值增大（P<0.01），与荷瘤1周组比较，荷瘤2周和3周组肿瘤中心PS值增大（P<0.05）；肿瘤区VEGF阳性表达随肿瘤生长而增加；肿瘤中心区CBV和PS值与VEGF表达呈正相关，rs均为0.453（P<0.05），肿瘤周边CBV和PS值与VEGF表达呈正相关，rs分别为0.443和0.742（P<0.05）。结论：大鼠C6脑胶质瘤的生长伴随着肿瘤不同区域CBV和PS的增加，肿瘤区CBV和PS值变化与VEGF表达密切相关。
Abstract
Objective: To evaluate cerebral blood volume(CBV) and permeability surface(PS) of rat C6 brain gliomas by CT perfusion and to study the correlation with VEGF expression. Methods: C6 cells were inoculated into the right caudate nucleus of 30 Wistar rats by stereotactic procedure to establish animal model. CT perfusion was performed on 1 week, 2 and 3 weeks after implantation. Immediately after each examination, some rats were sacrificed for pathology. Results: All CBV and PS values of the tumors at 1 week, 2 and 3 weeks after implantation increased compared to those of the contralateral sides(P<0.05). The PS value in the center of the tumor at 2 and 3 weeks after implantation were larger than that in the peripheral regions of the tumors(P<0.01). The PS value had statistical difference comparing with that of the tumors at 1 week and central tumoral regions both at 2 and 3 weeks after implantation(P<0.05). Expression of VEGF was observed adjacent to the vessel especially at the periphery of the tumors and increased gradually, positive correlation was present not only between central-tumor CBV and PS(rs=0.453, P<0.05) but also at both peripheral-tumor CBV and PS(rs=0.443 and 0.742 respectively, P<0.05) and VEGF expression under the development of the tumors. Conclusion: The blood volume and permeability of different tumor regions increase with the development of rat C6 brain gliomas, and perhaps there is a close correlation between both CBV and PS values and expression of VEGF.
关键词
脑肿瘤 /
神经胶质瘤 /
体层摄影术 /
X线计算机
Key words
Brain neoplasms /
Glioma /
Tomography /
X-ray computed
关丽明;徐 克;戚喜勋;方长兴;刘树立.
CT灌注扫描对脑胶质瘤的血流量和血管通透性及其与VEGF表达相关性的实验研究[J]. 中国临床医学影像杂志. 2008, 19(8): 547-550
GUAN Li-ming;XU Ke;QI Xi-xun;FANG Chang-xing;LIU Shu-li.
An experimental study of CT perfusion imaging in revealing the correlation ofcerebral blood volume, permeability and the VEGF expression[J]. Journal of China Clinic Medical Imaging. 2008, 19(8): 547-550
中图分类号：
Ｒ739.41
Ｒ730.264
Ｒ814.42
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