摘要
目的：探讨大鼠化学性肝纤维化的MR灌注加权成像（PWI）表现，评价PWI对肝纤维化的诊断价值。方法：皮下注射CCl4建立大鼠肝纤维化模型（50只）和对照组（10只）进行MR-PWI扫描。按肝纤维化病理分期进行分组，采用方差分析比较各组间门静脉和肝实质灌注曲线的峰值时间、信号最大上升斜率和最大下降斜率的变化，分析各灌注参数与肝纤维化程度的相关性。结果：模型组38只和对照组10只共48只大鼠完成MR检查。病理分期S0（n=13）、S1（n=5）、S2（n=9）、S3（n=13）、S4（n=8）。S0期门静脉和肝实质的峰值时间[（15.03±1.05）s和（25.93±1.70）s]，S1~S4期肝纤维化组的峰值时间逐渐递增[均值（26.29±3.52）s和（41.92±5.76）s]，S0期与S2~S4期差异有统计学意义（P<0.05，<0.001，<0.001）。S0期门静脉和肝实质的最大上升斜率、最大下降斜率[门静脉（420.46±27.26）1/s和（193.50±16.76）1/s，肝实质（140.10±16.73）1/s和（121.00±15.16）1/s]，S1~S4期肝纤维化组的最大上升、下降斜率逐渐递减[门静脉均值（292.61±40.02）1/s和（139.73±13.22）1/s，肝实质均值（76.70±9.55）1/s和（97.56±15.73）1/s]，S0~S2期与S3、S4期差异有统计学意义（P<0.05）。峰值时间与肝纤维化分期呈显著正相关（P<0.01），最大上升斜率、门静脉的最大下降斜率与肝纤维化分期呈显著负相关（P<0.01）。结论：MR-PWI可以检测大鼠肝纤维化改变，可对中重度（S2~S4期）肝纤维化进行早期诊断。
Abstract
Objective: To characterize liver perfusion in Wistar rats with experimental liver fibrosis by dynamic contrast-enhanced MRI and to correlate it with the severity of liver fibrosis. To evaluate the diagnostic efficacy of perfusion MR imaging for liver fibrosis. Methods: Mixed solution of CCl4 and olive oil was hypodermically injected to fifty Wistar rats in the experimental group. Ten healthy rats served as control. MR perfusion study with dynamic contrast-enhanced MRI was carried out on the rats under anesthesia. The rats were divided into S0~S4 stages according to the histological results of liver. Time to peak(TP), wash-in rate and wash-out rate was compared between the groups using variance analysis, and the relationship between each perfusion parameters and severity of liver fibrosis were made by correlative analysis. Results: Forty-eight rats underwent MR perfusion study, including ten rats in the normal group. Histological results S0（n=13）, S1（n=5）, S2（n=9）, S3（n=13）, S4（n=8）. On signal intensity time curve(SI-T) derived from MR perfusion weighted imaging(PWI), TP of portal vein and liver in fibrotic group[mean, (26.29±3.52)s and (41.92±5.76)s] increased progressively compared with control group[mean, (15.03±1.05)s and (25.93±1.70)s]. S2~S4 stage can be distinguished from S0 stage(P<0.05, <0.001, <0.001, respectively). Wash-in rate and wash-out rate of portal vein and liver in fibrotic group[mean, PV(292.61±40.02)1/s and (139.73±13.22)1/s, liver (76.70±9.55)1/s and (97.56±15.73)1/s] decreased progressively compared with control group[mean, PV(420.46±27.26)1/s and (193.50±16.76)1/s, liver(140.10±16.73)1/s and (121.00±15.16)1/s]. S3 and S4 stage can be distinguished from S0~S2 stage(P<0.05). TP value of the SI-T curve was significantly positive correlated to the severity of liver fibrosis(P<0.01). The wash-in rate and wash-out rate value were significantly negative correlated to the severity of liver fibrosis, respectively(P<0.01). Conclusion: MR-PWI has a potential in diagnosing and grading experimental liver fibrosis. PWI is an effective method to detect moderate and severe fibrosis(S2~S4 stage).
关键词
肝硬化 /
大鼠 /
动物实验 /
磁共振成像
Key words
Liver cirrhosis /
Rats /
Animal experimentation /
Magnetic resonance imaging
刘 鑫;郭启勇;廖 伟;王宝东.
肝纤维化磁共振灌注成像的初步实验研究[J]. 中国临床医学影像杂志. 2009, 20(6): 442-445
LIU Xin;GUO Qi-yong;LIAO Wei;WANG Bao-dong.
Assessment of liver fibrosis with perfusion-weighted MR imaging: preliminary study[J]. Journal of China Clinic Medical Imaging. 2009, 20(6): 442-445
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