USPIO增强MR黑血及白血成像序列检测兔动脉粥样硬化斑块的对比研究

李勇刚; 朱 默; 戴颖钰; 陈剑华; 倪健坤; 郭 亮

中国临床医学影像杂志 ›› 2010, Vol. 21 ›› Issue (3) : 179-182.

论著

USPIO增强MR黑血及白血成像序列检测兔动脉粥样硬化斑块的对比研究

李勇刚1，朱默1，戴颖钰1，陈剑华1，倪健坤2，郭亮1

作者信息 +

Comparison study between USPIO enhanced MRI black-blood sequence andwhite-blood sequence in the detection of atherosclerotic plaques in rabbits

LI Yong-gang1, ZHU Mo1, DAI Ying-yu1, CHEN Jian-hua1, NI Jian-kun2, GUO Liang1

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摘要

目的：探讨超小顺磁性氧化铁（USPIO）增强MR黑血及白血成像序列检测兔动脉粥样硬化（AS）斑块的敏感性及价值。方法：健康雄性新西兰大白兔30只，随机分为实验组20只，对照组10只。实验组采用球囊导管损伤腹主动脉内膜结合高脂饮食的方法建立兔动脉粥样硬化模型，对照组不作干预。MRI检查包括平扫、USPIO增强MRI黑血序列和白血序列。比较两种成像方法显示斑块形态、数目和成份的差异，与病理结果行对照研究，并做统计学分析。结果：12例兔AS模型成功建立，USPIO增强黑血序列T2WI及T2*WI扫描斑块中央信号减低，USPIO增强白血序列表现为管壁点状充盈缺损。扫描范围内普鲁士蓝染色阳性斑块共67个，MR黑血与白血序列对阳性斑块的检出率无显著性差异。黑血成像序列对于区分AS斑块成分较白血序列好。结论：USPIO颗粒可以被兔AS模型斑块中的巨噬细胞特异性吞噬并引起MRI信号的改变。USPIO增强MR黑血成像序列能敏感检出并反映兔AS斑块成分，有望用于对AS病变诊断及斑块稳定性的评估。

Abstract

Objective: To explore the sensitivity and value of ultrasmall superparamagnetic iron oxide(USPIO) enhanced MRI black-blood sequence and white-blood sequence in the detection of atherosclerotic plaques in rabbits. Methods: Thirty New Zealand male rabbits were divided randomly into two groups: 20 animals in the experiment group, 10 animals in control group. Animal model of atherosclerosis(AS) was induced with aortic balloon endothelial injury and high-fat diet feeding. There was no intervention to the rabbits in the control group. MRI examination included plain scan, USPIO enhanced black-blood sequence and white-blood sequence. The ability in demonstration of shape, number and component of the plaques between USPIO enhanced black-blood sequence and white-blood sequence was compared. A correlated study was done between MRI and pathological findings, as well as a statistical study. Results: Animal model of AS was successfully made in 12 rabbits. The central area of the plaques demonstrated a decreased signal on USPIO enhanced T2WI and T2*WI, but a dot-like filling defect on USPIO enhanced white-blood sequence. There were 67 plaques that were positive on Prussian blue staining within the MRI scanning scope. Although there was no difference in the detection of numbers of AS plaques between USPIO enhanced black-blood sequence and white-blood sequence, but the black-blood sequence was superior to the white-blood sequence in demonstrating of the components of plaques. Conclusion: USPIO could be phagocytosed by macrophages in the AS plaques of the rabbits, which could lead to the change of MR signal intensity. USPIO enhanced MR black-blood sequence can reveal the number and component of AS plaques and comply in the diagnosis and evaluation of the stability of AS plaques.

导出引用

李勇刚;朱默;戴颖钰;陈剑华;倪健坤;郭亮. USPIO增强MR黑血及白血成像序列检测兔动脉粥样硬化斑块的对比研究[J]. 中国临床医学影像杂志. 2010, 21(3): 179-182

LI Yong-gang;ZHU Mo;DAI Ying-yu;CHEN Jian-hua;NI Jian-kun;GUO Liang. Comparison study between USPIO enhanced MRI black-blood sequence andwhite-blood sequence in the detection of atherosclerotic plaques in rabbits[J]. Journal of China Clinic Medical Imaging. 2010, 21(3): 179-182

中图分类号： Ｒ543.3 Ｒ445.2