脑星型细胞瘤磁共振波谱与肿瘤细胞的Ki-67、bcl-2相关性研究

苗红; 刘文源; 宋福林

中国临床医学影像杂志 ›› 2011, Vol. 22 ›› Issue (6) : 389-393.

论著

脑星型细胞瘤磁共振波谱与肿瘤细胞的Ki-67、bcl-2相关性研究

苗红1，刘文源2，宋福林2

作者信息 +

MR spectroscopy of human astrocytoma and the correlation with the expression of Ki-67 and bcl-2

MIAO Hong1, LIU Wen-yuan2, SONG Fu-lin2

Author information +

文章历史 +

摘要

目的：探讨脑星形细胞瘤磁共振波谱（MRS）与肿瘤细胞的细胞核增殖抗原（Ki-67）蛋白、bcl-2蛋白表达的相关性。方法：对照观察52例脑星形细胞瘤MRS检查结果与免疫组化染色检测术后标本的Ki-67和bcl-2蛋白表达，分析肿瘤实质内NAA/Cho、Cho/Cr和NAA/Cr值与肿瘤级别的关系，并分别与Ki-67、bcl-2进行相关性分析。结果：脑星形细胞瘤的Cho/NAA、Cho/Cr、NAA/Cr比值与病理级别相关。Ki-67和bcl-2在星形细胞瘤中有阳性表达，且随着脑星形细胞瘤病理分级程度的升高而升高（r=0.600，P<0.01）。星形细胞瘤Cho/Cr与bcl-2蛋白表达呈明显正相关（r=0.736，P<0.01）；星形细胞瘤NAA/Cho与bcl-2蛋白表达呈明显负相关（r=-0.253，P<0.01）。星形细胞瘤Cho/Cr与Ki-67蛋白表达呈明显正相关（r=0.809，P<0.01），星形细胞瘤NAA/Cho与Ki-67蛋白表达呈明显负相关（r=-0.501，P<0.01），星形细胞瘤NAA/Cr与bcl-2蛋白表达呈负相关（r=-0.369，P<0.01），星形细胞瘤NAA/Cr与Ki-67呈负相关（r=-0.416，P<0.01）。结论：MRS基本反映着肿瘤细胞Ki-67及bcl-2表达情况，可在术前预测肿瘤代谢特性、增殖潜能、凋亡抑制情况、侵袭能力和预后。其中NAA/Cho和Cho/Cr比值反映肿瘤级别较稳定，较NAA/Cr更有意义。

Abstract

Objective: To investigate the relationship between the MRS and the Ki-67, bcl-2 expression of human astrocytoma. Methods: Fifty-two patients of astrocytoma proved by surgery and pathology were included. MRS was performed in all the patients. After surgical operation, the specimen was examined for Ki-67 and bcl-2. The NAA/Cho, Cho/Cr and NAA/Cr were recorded and the relationship with tumor grade was analyzed. The relationship between the above indices and Ki-67, bcl-2 were investigated respectively. Results: There was significant correlation between NAA/Cr, Cho/Cr, NAA/Cho and the pathological grading of astrocytoma. Positive expression of Ki-67 and bcl-2 in astrocytoma. Positve expression of Ki-67, bcl-2 increased with increasing clinicopathologic grades(r=0.600, P<0.01). The Cho/Cr was strongly positively correlated with expression of bcl-2 and Ki-67(r=0.736, P<0.01; r=0.809, P<0.01 respectively). The NAA/Cho was markedly negative correlated with the expression of bcl-2(r=-0.253, P<0.01), and had markedly negative correlation with Ki-67(r=-0.501, P<0.01). The NAA/Cr of astrocytoma was negatively related with the expression of bcl-2(r=-0.369, P<0.01) and Ki-67(r=-0.416, P<0.01). Conclusion: MRS can reflect the protein manifestation of Ki-67 and bcl-2 of astrocytoma noninvasively, being helpful in the preoperative assessment of apoptosis, metabolic information, reproductive activity, invasive activity and can predict prognosis of astrocytomas. The Cho/Cr, NAA/Cho reflected pathologic grade of astrocytoma relatively steadily. The Cho/Cr and NAA/Cho are more useful than NAA/Cr.

导出引用

苗红;刘文源;宋福林. 脑星型细胞瘤磁共振波谱与肿瘤细胞的Ki-67、bcl-2相关性研究[J]. 中国临床医学影像杂志. 2011, 22(6): 389-393

MIAO Hong;LIU Wen-yuan;SONG Fu-lin. MR spectroscopy of human astrocytoma and the correlation with the expression of Ki-67 and bcl-2[J]. Journal of China Clinic Medical Imaging. 2011, 22(6): 389-393

中图分类号： Ｒ739.41 Ｒ730.264 Ｒ445.2