18F-FDG与人卵巢癌细胞株SKOV3结合实验方法学研究

刘文平; 吴翼伟; 章 斌; 邓胜明; 崔 凤; 徐坤元

中国临床医学影像杂志 ›› 2011, Vol. 22 ›› Issue (8) : 542-546.

论著

18F-FDG与人卵巢癌细胞株SKOV3结合实验方法学研究

刘文平1，吴翼伟2，章斌2，邓胜明2，崔凤1，徐坤元1

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Studies on the methodology of 18F-FDG cell binding of ovarian cancer SKOV3

LIU Wen-ping1, WU Yi-wei2, ZHANG Bin2, DENG Sheng-ming2, CUI Feng1, XU Kun-yuan1

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摘要

目的：建立18F-FDG与人卵巢癌细胞株SKOV3结合实验的方法学。方法：在不同条件下测定18F-FDG与人卵巢癌细胞株SKOV3结合率。①细胞数分别为5×104个/瓶、1×105个/瓶、5×105个/瓶、1×106个/瓶、5×106个/瓶；②反应时间分别为20min、40min、60min、80min、100min和120min；③18F-FDG放射性活度分别为1.85KBq、3.7KBq、7.4KBq、14.8KBq和29.6KBq；④葡萄糖的浓度分别为0mmol/L、2.78mmol/L、5.55mmol/L和11.1mmol/L。用γ测量仪测量细胞的CPM（B）及上清液CPM（F）。计算18F-FDG的细胞结合率=[B/（B+F）]×100%。结果：①18F-FDG与人卵巢癌细胞株SKOV3结合率（y）与细胞数（x1）、反应时间（x2）、葡萄糖的浓度（x3）存在线性回归关系，回归方程：y=（7.395+5.18E-0.06x1+0.094x2-2.040x3）×100%，F=106.9，P<0.05。偏回归系数P均<0.05。校正决定系数R2c=0.728，P<0.05。细胞结合率与细胞数、反应时间、葡萄糖的浓度Pearson相关系数分别为0.581、0.179、-0.599，P均<0.05。②18F-FDG与人卵巢癌细胞株SKOV3结合实验基本条件：细胞数量为1×106个/瓶，18F-FDG放射性活度3.7KBq，反应时间100min，葡萄糖浓度0mmol/L，细胞结合率为（25.45±1.66）%。结论：建立了18F-FDG与人卵巢癌细胞株SKOV3结合实验的方法学，为18F-FDG结合实验早期评价放疗和化疗对人卵巢癌细胞株SKOV3的抑制作用奠定了基础。

Abstract

Objective: To establish a useful and stable methodology of 18F-FDG cell binding of ovarian cancer SKOV3. Methods: Several binding conditions were tested. ①The cell number was from 5×104 to 5×106 cells/bottle. ②The reaction time was from 20 to 120min. ③The radioactivity of 18F-FDG was from 1.85 to 29.6KBq. ④The glucose concentration was from 0 to 11.1mmol/L. The CPM(B) in cells and CPM(F) in supernatant were measured by the γ detector. The binding efficiency of ovarian cancer cell line SKOV3 with 18F-FDG was calculated. Results: ①There was linear regression relationship between 18F-FDG cell binding efficiency of human ovarian cancer SKOV3(y) and the cell number(x1), reaction time(x2), glucose concentration(x3). Regression equation: y=(7.395+5.18E-0.06x1+0.094x2-2.040x3)×100%, F=106.9, P<0.05. The P values of partial regression coefficient were less than 0.05. R2c=0.728, P<0.05. The Pearson correlation coefficient of cell number, reaction time, glucose concentration with 18F-FDG cell binding efficiency was 0.581、0.179、-0.599 respectively, P<0.05. ②The basic conditions of 18F-FDG cell binding of ovarian cancer SKOV3: the cell number was 1×106 cells/bottle, the radioactivity of 18F-FDG was 3.7KBq, the glucose concentration was 0mmol/L, the reaction time was 100min. The binding efficiency was (25.45±1.66)%. Conclusions: The methodology of 18F-FDG cell binding of ovarian cancer SKOV3 was established successfully. It contributed to evaluate early the effect of radiotherapy and chemotherapy on inhibiting ovarian cancer cell line SKOV3.

导出引用

刘文平;吴翼伟;章斌;邓胜明;崔凤;徐坤元. 18F-FDG与人卵巢癌细胞株SKOV3结合实验方法学研究[J]. 中国临床医学影像杂志. 2011, 22(8): 542-546

LIU Wen-ping;WU Yi-wei;ZHANG Bin;DENG Sheng-ming;CUI Feng;XU Kun-yuan. Studies on the methodology of 18F-FDG cell binding of ovarian cancer SKOV3[J]. Journal of China Clinic Medical Imaging. 2011, 22(8): 542-546

中图分类号： R737.31 R817.4