摘要
目的：探讨经肾动脉化疗栓塞术（TACE）对兔VX2肾移植瘤模型增殖细胞核抗原（PCNA）表达的影响，评价兔VX2肾移植瘤介入治疗的疗效。材料与方法：将瘤块种植到22只新西兰纯种大白兔的左肾上极。超声监测肿瘤生长，待肿瘤长至1~2 cm大小时，将实验兔随机分为对照组、TACE组，对照组10只，实验组12只。行TACE术：对照组（A组）给予生理盐水10 mL；TACE组（B组）给予超液化碘油0.2 mL/kg和阿霉素2 mg/kg。栓塞后7 d处死动物取出瘤块，用10%中性福尔马林液固定，石蜡包埋，5 μm连续切片，免疫组化染色检测肿瘤PCNA的表达。结果：本实验免疫组化结果显示2组共22只实验动物中PCNA均有表达，阳性率为100%，TACE组（71.6±11.01）%的细胞增殖指数（PI）明显高于对照组（61.2±11.21）%（P=0.041）。结论：经导管动脉化疗栓塞术是一种创伤小、疗效确切的兔VX2肾移植瘤治疗方法；TACE术后1周残存肿瘤组织细胞PI升高。
Abstract
Objective: To investigate the therapeutic effect and the expression of proliferating cell nuclear antigen(PCNA) in renal implantation tumor after transcatheter arterial chemoembolization(TACE). Methods: VX2 tumor was inoculated directly into the exposed kidney by tumor blocks embedding in twenty-two New Zealand white rabbits. When the diameter of tumors reached 1~2 cm, the rabbits bearing tumor were randomly divided into two groups, control group(group A n=10) and experimental group(group B n=12). For group A, normal saline was administered via hepatic artery. For group B, lipiodol(0.2 mL/kg) and ADM(2 mg/kg) were administered for each rabbit. Seven days after embolism, the rabbits were sacrificed and the tumors were taken out. All specimens were fixed by 10% neutral formalin, embedded with paraffin completely and consecutive sections were made with a 5 μm-thickness. Then immunohistochemistry was performed to demonstrate the expression of PCNA. Results: PCNA was expressed in 22 animals in two groups(the postive rate=100%). The PI (71.6±11.01)% of residual tumor cells of group B was higher than that of group A (61.2±11.21)%(P<0.05). Conclusions: TACE is a minor trauma and effective treatment for rabbits VX2 renal tumor, the proliferation of residual tumor cells is increased effectually in experimental group.
关键词
肾肿瘤 /
兔 /
增殖细胞核抗原 /
栓塞 /
治疗性
Key words
Kidney neoplasms /
Rabbits /
Proliferating cell nuclear antigen /
Embolization /
therapeutic
刘 峰;柳 港;王 帅;王 滨;蹇兆成;孙业全.
TACE治疗对兔VX2肾移植瘤细胞增殖影响的实验研究[J]. 中国临床医学影像杂志. 2012, 23(3): 181-183
LIU Feng;LIU Gang;WANG Shuai;WANG Bin;JIAN Zhao-cheng;SUN Ye-quan.
Experimental studies on cell proliferation after transcatheter arterial chemoembolization ofVX2 carcinoma implanted in rabbit kidney[J]. Journal of China Clinic Medical Imaging. 2012, 23(3): 181-183
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