摘要
目的：探讨氧气刺激的血氧水平依赖（BOLD）磁共振成像ΔR2*值与大鼠肝纤维化程度的关系，初步探讨应用ΔR2*值评估肝纤维化的潜在价值。方法：腹腔注射CCl4法建立大鼠肝纤维化模型（90只）和对照组（10只）进行吸氧前、后的BOLD磁共振成像，测算吸氧前后肝脏R2*值的变化量（ΔR2*）。以肝纤维化病理分期为基础将其划分为：A组：无纤维化组（S0），B组：轻中度纤维化组（S1~S2），C组：重度纤维化/肝硬化组（S3~S4），采用单因素方差分析比较各组间的ΔR2*值的差异，采用非参数Spearman检验分析ΔR2*值与病理肝纤维化程度的相关性。结果：模型组32只和对照组10只共42只大鼠完成MR检查。大鼠吸氧后R2*绝对值不同程度减低，A组、B组及C组的平均ΔR2*值分别为（24.05±4.65） Hz、（17.18±4.50） Hz、（8.09±5.17） Hz，经统计学分析，A组、B组、C组的ΔR2*值差异均有统计学意义（P<0.05）；ΔR2*值随肝纤维化程度的加重逐渐减小，与纤维化程度呈负相关（r=-0.827，P<0.001）。结论：氧气刺激的BOLD磁共振成像的ΔR2*值可反映纤维化进程中肝脏血流动力学及血氧含量的综合变化，在肝纤维化的检出及严重程度评价中有潜在的应用价值。
Abstract
Objective: To explore the relationship between ΔR2* value in oxygen-challenge blood oxygen level-dependent(BOLD) MRI and the severity of liver fibrosis in a rat model, and assess the potential value of diagnosis of the disease using ΔR2* value. Methods: Liver fibrosis was induced in 90 rats by means of weekly intraperitoneal injection with a mixture of carbon tetrachloride and olive oil. Ten healthy rats were used as controls. BOLD-MRI study was performed on the rats under anesthesia at steady normoxia state by inhaling room air and hyperoxia state by inhaling oxygen. The changes in R2* values(ΔR2*) during the oxygen challenge were quantified on the post-processing workstation. According to the pathological results, the rats were divided into three groups: A: without fibrosis(S0), B: mild and moderate fibrosis(S1~S2), C: severe fibrosis and cirrhosis(S3~S4). The differences of ΔR2* among the groups were tested by ANOVA and the correlation between liver fibrosis degree and ΔR2* were analyzed by Spearman-test. Results: Thirty-two liver fibrosis model rats and 10 healthy rats were performed BOLD-MRI. Absolute values of R2* decreased after oxygen challenge in all rats. A statistically significance(P<0.05) in ΔR2* were observed between group A((24.05±4.65) Hz), group B ((17.18±4.50) Hz) and group C((8.09±5.17) Hz). ΔR2* decreased during liver fibrosis progression and was negatively correlated with liver fibrosis degree(r=-0.827, P<0.001). Conclusion: ΔR2* in oxygen-challenge BOLD-MRI can reflect hemodynamic and oxygenation alterations during the progression of liver fibrosis, and has the potential to serve as a noninvasive method for liver fibrosis diagnosis and severity evaluation.
关键词
肝硬化 /
磁共振成像 /
大鼠
Key words
Liver cirrhosis /
Magnetic resonance imaging /
Rats
张 兰;石 喻;卞胜昕;郭启勇.
氧气刺激的BOLD磁共振成像ΔR2*值评估鼠肝纤维化的初步实验研究[J]. 中国临床医学影像杂志. 2013, 24(3): 174-177
ZHANG Lan;SHI Yu;BIAN Sheng-xin;GUO Qi-yong.
Assessment of rat liver fibrosis with ΔR2* value in oxygen-challenge BOLD MR imaging: a preliminary study[J]. Journal of China Clinic Medical Imaging. 2013, 24(3): 174-177
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