耐药人乳腺癌细胞株Bcap37/MDR1及其亲本细胞株Bcap37 18F-FDG细胞结合实验的比较研究

肖汉; 张玮; 章斌; 邓胜明; 周海中

中国临床医学影像杂志 ›› 2013, Vol. 24 ›› Issue (8) : 551-554.

论著

耐药人乳腺癌细胞株Bcap37/MDR1及其亲本细胞株Bcap37 18F-FDG细胞结合实验的比较研究

肖汉1，张玮2，章斌2，邓胜明2，周海中1

作者信息 +

Comparative studies of the 18F-FDG cell binding the drug-resistant breast cancer cell Bcap37/MDR1 and breast cancer cell Bcap37

XIAO Han1, ZHANG Wei2, ZHANG Bin2, DENG Sheng-ming2, ZHOU Hai-zhong1

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文章历史 +

摘要

目的：对耐药人乳腺癌细胞株Bcap37/MDR1与其亲本细胞株Bcap37 18F-FDG结合率进行比较，为进一步研究化疗药物对两细胞株抑制的实验研究奠定基础。方法：在不同条件下分别测定18F-FDG与Bcap37/MDR1和Bcap37的结合率。①细胞数为1.25×105~1×107个/瓶；②反应时间为20~120 min；③18F-FDG放射性活度为1.85~29.6 KBq；④葡萄糖的浓度为0~5.5 mmol/L。用γ测量仪测量细胞的CPM计数（B）及上清液CPM计数（F），计算18F-FDG的细胞结合率（%）=（B/（B+F））×100%，并行两者差异的比较。结果：当其它条件不变，分别改变细胞数量、反应时间、18F-FDG放射性活度时，Bcap37结合率均高于Bcap37/MDR1，两组差异均有统计学意义（P＜0.05）。当其它条件不变，葡萄糖的浓度为0 mmol/L和1.39 mmol/L时，两组细胞间结合率差异均有统计学意义（P＜0.05）；葡萄糖的浓度为2.78 mmol/L和5.5 mmol/L时，两组细胞间结合率差异均无统计学意义（P＞0.05）。结论：在各种实验条件下，Bcap37/MDR1的18F-FDG细胞结合率均低于Bcap37，表明耐药肿瘤细胞的增殖及代谢均低于其亲本肿瘤细胞。

Abstract

Objective: To compare the 18F-FDG cell binding in the drug-resistan breast cancer cell Bcap37/MDR1 and breast cancer cell Bcap37, to lay the foundation for a further study of the inhibitory effect of chemotherapy in breast cancer cell Bcap37/MDR1 and breast cancer cell Bcap37. Methods: Several different binding conditions were tested. ①The cell number was 1.25×105~1×107/bottle. ②The reaction time was 20~120 min. ③The radioactivity of 18F-FDG was 1.85~29.6 KBq. ④The glucose concentration was 0~5.5 mmol/L. The count of CPM(B) in cells and CPM(F) in supernatant were measured by the γ equipment. The binding efficiency of Bcap37 and Bcap37/MDR1 cell with 18F-FDG were calculated and compared. Result: When other conditions were invariable, and separately changed cell number, the reaction time and the 18F-FDG radioactivity, the binding efficiency of Bcap37 was higher than the Bcap37/MDR1. The difference was significant(P<0.05). The difference of binding efficiency between Bcap37 and Bcap37/MDR1 cell at the 0 and 1.39 mmol/L groups was significant(P<0.05); The difference of binding efficiency between Bcap37 and Bcap37/MDR1 cell at 2.78 mmol/L and 5.5 mmol/L groups was not statistically significant(P>0.05). Conclusions: The 18F-FDG cell binding of breast cancer Bcap37/MDR1 was lower than the Bcap37. It showed that the proliferation and metabolism of drug resistance of tumor cells were lower than the parental tumor cells.

导出引用

肖汉;张玮;章斌;邓胜明;周海中. 耐药人乳腺癌细胞株Bcap37/MDR1及其亲本细胞株Bcap37 18F-FDG细胞结合实验的比较研究[J]. 中国临床医学影像杂志. 2013, 24(8): 551-554

XIAO Han;ZHANG Wei;ZHANG Bin;DENG Sheng-ming;ZHOU Hai-zhong. Comparative studies of the 18F-FDG cell binding the drug-resistant breast cancer cell Bcap37/MDR1 and breast cancer cell Bcap37[J]. Journal of China Clinic Medical Imaging. 2013, 24(8): 551-554

中图分类号： Ｒ737.9 Ｒ817.4