摘要
目的：观察射频消融治疗兔肝VX2肿瘤后不同时期残余瘤的超声表现，探讨残余瘤组织中血管内皮生成因子（VEGF）及微血管密度（MVD）的表达变化。方法：建立兔VX2肝癌模型，超声引导下控制RFA术肿瘤消融半径，建立兔VX2残癌模型。于治疗前及治疗结束后0 h、1周、2周、4周行超声观察后切取标本，采取免疫组织化学法观察残癌组织中VEGF及MVD的表达及变化。结果：①超声显示术后1周、2周时，消融灶变小、血流信号减少；术后4周消融灶略增大，血流信号复现。②RFA术后0 h、1周、2周残癌组织中VEGF和MVD的表达较术前降低（P＜0.05）；术后4周残癌组织中VEGF和MVD表达较术前及前3组增加（P＜0.05）。结论：短期内（2周）能破坏肿瘤血管并抑制残癌组织血管形成从而达到减瘤疗效，但长期缺氧可诱导新生血管形成，残余瘤复发。
Abstract
Objective: To observe the sonographic findings of the residual tumor in different time intervals after radiofrequency ablation(RFA) treatment to rabbit VX2 liver tumor, and to study the changes of vascular endothelial grow factor(VEGF) and the expression of microvascular density(MVD) in the residual tumor tissue. Method: The rabbit VX2 liver cancer model was established, and radius of the radiofrequency was controlled under ultrasound guidance, then the residual VX2 liver tumor in rabbit was established. The specimens before and after treatment at 0 h, 1 w, 2 w, 4 w were acquired for ultrasonic observation. It was to observe the expression of VEGF and MVD in the residual cancer tissue by the immune histochemical method. Results: ①Both the 1 w and 2 w residual tumor were smaller and the vascularization were lesser than those of the 4 w specimen. The signal of vascularization reappeared at 4 w specimen. ②Compared with the tumor before RFA, the expression of VEGF and MVD in the residual tumor tissues at 0 h, 1 w, 2 w decreased(P<0.05). However compared with the tumor before RFA and the residual tumor tissues at 0 h, 1 w, 2 w, the expression of VEGF and MVD in 4 w specimen increased(P<0.05). Conclusion: In the short term(within 2 w) RFA destroys tumor vessels thus controls and inhibits VEGF to obtain short term curative effect, but in the long term can induce neovascularization due to hypoxia and tumor recurrency occurred.
关键词
肝肿瘤 /
肿瘤 /
残余 /
超声检查 /
多普勒 /
彩色 /
兔
Key words
Liver neoplasms /
Neoplasm /
residual /
Ultrasonography /
Doppler /
color /
Rabbits
王健琳;刘 颖;范秋阳;马艳艳;张璟琸;邱天爽.
兔肝VX2肿瘤RFA术后残余瘤的超声表现及新生血管的研究[J]. 中国临床医学影像杂志. 2013, 24(9): 639-643
WANG Jian-lin;LIU Ying;FAN Qiu-yang;MA Yan-yan;ZHANG Jing-zhuo;QIU Tian-shuang.
Sonographic findings of the neovascularization in the retained liver VX2 tumor ofrabbit after radiofrequency ablation: an experimental study[J]. Journal of China Clinic Medical Imaging. 2013, 24(9): 639-643
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