摘要
目的：探讨13N-NH3·H2O PET/CT在大鼠肝纤维化早期定量化诊断中的应用价值。方法：对照组大鼠5只；肝脏纤维化模型实验组12只，其中S1期（轻度肝纤维化组）7只，S2+S3+S4期（进展期及重度肝纤维化组）5只。以13N-NH3·H2O作为显像剂，乙酰唑胺作为水通道蛋白抑制剂，分别对实验组和对照组大鼠进行动态PET/CT扫描，进行数据重建及图像分析，通过比较不同显像时间点（20 s、90 s、5 min、10 min、15 min、20 min和25 min）正常对照组大鼠与肝纤维化模型大鼠肝脏对13N-NH3·H2O的摄取率，比较各组大鼠在血流灌注、组织扩散及13N-NH3·H2O代谢的差异。结果：自身配对t检验S0在10 min、15 min时点，S1在20 s时点，S2+S3+S4在10 min、15 min、20 min、25 min时点使用抑制剂前后的SUV有统计学差异。各组间各时点SUV无明显统计学差异。结论：13N-NH3·H2O PET/CT联合乙酰唑胺抑制实验能够诊断早期肝纤维化并对肝纤维化进行分期。
Abstract
Objective: To investigate the value of 13N-NH3·H2O PET/CT in quantitative diagnosis of early liver fibrosis. Methods: Using 13N-NH3·H2O as imaging agent and acetazolamide(ACZ) as inhibitor, 5 rats of control group and 12 rats of liver fibrosis(7 of stage S1 and 5 of stage S2+S3+S4) undertook dynamic PET/CT scan. With data reconstruction and imaging analysis, we calculated the uptake rate of 13N-NH3·H2O by livers in the two groups at time points of 20 s, 90 s, 5 min, 10 min, 15 min, 20 min and 25 min. Blood perfusion, interstitial diffusion and 13N-NH3·H2O uptake were compared between the two groups at different time points. Results: The SUV of S0 at time points of 10 min and 15 min, S1 at 20 s, S2+S3+S4 at 10 min, 15 min, 20 min and 25 min showed statistical difference before and after using the inhibitor(ACZ). The SUV of each group at any time point had no obvious difference. Conclusion: 13N-NH3·H2O PET/CT combined with ACZ can diagnose early liver fibrosis and staging of liver fibrosis.
关键词
肝硬化 /
正电子发射断层显像术 /
体层摄影术 /
X线计算机 /
大鼠
Key words
Liver cirrhosis /
Positron-emission tomography /
Tomography /
X-ray computed /
Rats
韩婷婷;杜 名;张 新;曹 礼;李 红;赵周社;辛 军;郭启勇.
13N-NH3·H2O PET/CT在大鼠肝纤维化模型成像的定量研究[J]. 中国临床医学影像杂志. 2014, 25(4): 243-247
HAN Ting-ting;DU Ming;ZHANG Xin;CAO Li;LI Hong;ZHAO Zhou-she;XIN Jun;GUO Qi-yong.
Quantitative analysis of early liver fibrosis in rats by 13N-NH3·H2O PET/CT[J]. Journal of China Clinic Medical Imaging. 2014, 25(4): 243-247
中图分类号：
Ｒ575.2
Ｒ817.4
Ｒ814.42
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