目的：在常规磁共振成像仪上进行小型动物实验，探讨昆明小鼠MRI T1平扫及普美显动态增强扫描最佳成像参数，为小型动物肝脏影像学实验研究提供参考。方法：采用西门子3.0T超导MR成像仪，腕关节专用线圈。选取雄性昆明小鼠10只，6~8 W，分别进行T1WI及T1动态增强扫描，动态增强不间断扫描10期。尾静脉注射，普美显用生理盐水稀释100倍，剂量采用0.05 mL/10g、0.1 mL/10g、0.15 mL/10g。T1WI平扫及动态增强后处理形成时间与信号强度百分比曲线，观察曲线的形态。测量肝脏T1WI的信号强度、增强后胆囊显影时肝脏的信号强度及肝脏与肌肉信号的组织对比度（平扫及增强），进行比较及统计学分析；计算出肝实质信号的强化率，并与肌肉信号的强化率进行比较。结果：尾静脉注射造影剂剂量采用0.1 mL/10g，动态增强时间与信号强度百分比曲线呈上升平台型，第4期之后呈平台型，胆囊显影在第5期或第6期。小鼠肝脏平扫与增强的信号强度比较有明显统计学差别；肝脏及肌肉平扫信号强度组织对比度与肝脏及肌肉增强扫描信号强度组织对比度比较有明显统计学差别；肝实质信号的强化率与肌肉信号的强化率比较亦有明显统计学差异。结论：在常规MRI上进行小型动物实验切实可行，可以进行小鼠肝脏普美显动态增强扫描。普美显动态增强扫描曲线呈上升平台型，胆囊显影清晰，可以利用普美显进行相关肝脏疾病的研究。

Objective: To investigate the optimal imaging parameters of MRI T1 plain scan and Gd-EOB-DTPA dynamic enhanced scan in kunming mice based on small animal experiments on conventional MRI, to provide references for the experimental study of small animal liver imaging. Methods: Ten male kunming mice(6~8 W) were performed with T1WI and T1 dynamic enhanced scanning(10-phase dynamic enhanced continuous scanning) using Siemens 3.0T MRI and special coil of wrist joint and tail vein injection. Gd-EOB-DTPA was diluted 100 times with saline. Dosage: 0.05 mL/10g, 0.1 mL/10g, 0.15 mL/10g. The time and signal intensity percentage curve was formed after T1WI plain scan and dynamic enhancement, and the shape of the curve was observed. The T1WI signal intensity of the liver, the signal intensity of the liver at the time of the development of the gallbladder and the tissue contrast of the liver and muscle signal were measured and analyzed. The enhancement rates of the liver parenchyma signal were calculated and compared with the muscle signal enhancement rates. Results: The dose of tail vein injection contrast agent was 0.1 mL/10g, and the time and signal intensity percentage curve was rising platform type, and followed by the platform type after the fourth stage, and the gallbladder developed at fifth or sixth stage. There were significant differences of the signal intensity between plain scans and enhanced scans in small animal livers, and of the signal enhancement rate between the liver parenchyma and the muscle. The contrast of the plain scan signal intensity of the liver to the muscle was significantly different from the contrast of the enhanced signal intensity of the liver. Conclusion: Small animal experiments on conventional MRI were feasible. The Gd-EOB-DTPA dynamic enhanced scan in kunming mice was also feasible. The curve of the Gd-EOB-DTPA dynamic enhanced scan showed rising platform type. The developing of gallbladder was clear. The Gd-EOB-DTPA can be used for the research of the related liver diseases.