目的：探讨肝细胞性肝癌（HCC）中突变型P53蛋白表达与超声造影定量分析参数的相关性。方法：回顾性分析57例HCC患者超声造影定量分析的各项参数，取得肿瘤组织并用免疫组化染色的方法测定P53蛋白的表达，分析它与造影定量分析参数的相关性。结果：①P53的阳性表达率为54.3%（31/57）。②在P53阳性表达组和阴性表达组中峰值强度、平均渡越时间、达峰时间之间无显著差异（P>0.05），而上升斜率、流出时间之间有显著差异（P<0.05）。③P53的阳性表达与峰值强度、平均渡越时间、达峰时间无明显相关性（P>0.05）；P53的阳性表达与上升斜率呈正相关，r=0.377（P<0.05），与流出时间呈负相关， r=-0.410（P<0.05）。结论：HCC超声造影参数的上升斜率、流出时间与突变型P53蛋白的表达之间存在一定的相关性，通过时间-强度曲线，可以无创性的评估P53的表达。
Abstract
Objective: To investigate the expression of mutant P53 protein and their correlation with contrast-enhanced ultrasound quantitative parameters in hepatocellular carcinoma(HCC). Methods: Fifty-seven cases of HCC were enrolled. A retrospective analysis of the contrast-enhanced ultrasound quantitative parameters was performed, and then their correlation with P53 protein by immunohistochemistry staining method was done. Results:①P53 positive expression rate was 54.3%(31/57). ②In P53 positive and negative groups, the peak strength, the mean transit time and time to peak showed no significant difference(P>0.05). There was significant difference in the rising slope and washout time(P<0.05). ③There was no correlation between the expression of P53 and maximum intensity, mean transit time or time to peak(P>0.05). The positive expression of P53 was positively correlated with the rising slope with r=0.377(P<0.05), and negatively correlated with washout time with r=-0.410(P<0.05). Conclusion: For HCC contrast-enhanced ultrasound parameters, there is a certain correlation between rising slope, washout time and mutant P53 protein expression. The time-intensity curve might noninvasively assess the expression of P53.
关键词
肝肿瘤 /
癌 /
肝细胞 /
基因 /
p53 /
超声检查 /
多普勒 /
彩色
Key words
Liver neoplasms /
Carcinoma, hepatocellular /
Genes, p53 /
Ultrasonography, Doppler, color
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基金
国家自然科学基金（81260222）；广西自然科学基金（2012GXNSFAA276041）；广西卫生厅重点科研项目（桂卫重 2011089）。
