目的：利用MR灌注成像（MR PWI）评价瑞加德松对兔脑血脑屏障的开放程度。方法：50只新西兰大白兔随机分为实验组（A组）和对照组（B组），分别经耳缘静脉注入瑞加德松和生理盐水，延迟10 min后行MR PWI扫描。比较分析两组感兴趣区rCBF、rCBV、TTP及MTT参数值差异（配对t检验）。各组随机选5只兔子，图像采集后即刻静脉注入2%伊文氏蓝，1小时后处死取脑组织观察蓝染情况。结果：Ａ组感兴趣区rCBF、rCBV较B组明显增高，脑组织蓝染明显，有统计学差异（P<0.05），TTP值、MTT值在两组间无明显差异（P＞0.05）。结论：MR PWI的参数rCBF和rCBV可在体监测瑞加德松开放血脑屏障的程度。
Abstract
Objective: To evaluate the level of regadenoson-induced blood-brain barrier opening of healthy rabbits via MR perfusion weighted imaging(MR PWI) scan. Methods: Fifty New Zealand white rabbits were randomly divided into experimental group(group A) and control group(group B). The regadenoson and normal saline were injected intravenously into ear margin separately, after 10 minute, MR PWI scan was performed. Then rCBF, rCBV, TTP and MTT of the region of interest of each group were acquired and compared statistically(paired t test). Five cases of each group choosed randomly were injected with 2% Evens blue intravenously posterior to imaging. After one hour of infusion, all the animals were killed. Their brains were examined for the determination of Evens blue distribution. Results: The rCBF and rCBV values and the staining with Evens blue of group A were significantly higher than those of group B(P<0.05). There were no significant differences of TTP and MTT values between group A and B(P>0.05). Conclusion: The parameters(rCBF, rCBV) of MR PWI scan can monitor the level of regadenoson-induced blood-brain barrier opening in vivo.
关键词
血脑屏障 /
磁共振成像
Key words
Blood-brain barrier /
Magnetic resonance imaging
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基金
本文课题受辽宁省科技攻关基金项目（编号：2012225019）资助。
