水通道蛋白磁共振分子成像与水通道蛋白4表达的相关性研究

陈秋雁; 吴富淋; 彭晓澜; 李春丽; 江  敏; 陈婷婷; 魏鼎泰

中国临床医学影像杂志 ›› 2016, Vol. 27 ›› Issue (12) : 837-841.

论著

陈秋雁，吴富淋，彭晓澜，李春丽，江敏，陈婷婷，魏鼎泰

作者信息 +

Research on correlation between aquaporin magnetic resonance molecular imaging and AQP4 expression

CHEN Qiu-yan, WU Fu-lin, PENG Xiao-lan, LI Chun-li, JIANG Min, CHEN Ting-ting, WEI Ding-tai

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文章历史 +

摘要

目的：探讨水通道蛋白磁共振分子成像（AQP MRI）与水通道蛋白4（AQP4）表达量的相关性。方法：10只成年雄性SD大鼠随机分为两组，分别经单纯的脑缺血模型及肢体远端缺血（LRP）预处理（LRP组）或阴性预处理（Stroke组）后经历1 h的大脑中动脉栓塞建立脑缺血模型。在恢复再灌注后48 h行MRI检查采集AQP MRI图像，并行Western blot检测AQP4表达量。结果：10只大鼠最终符合建模成功条件的为6只，建模成功率为60%。AQP MRI显示1~5号大鼠患侧的AQP ADC值较健侧降低，而6号大鼠患侧的AQP ADC值较健侧显著升高。Western blot结果显示1~6号大鼠患侧的AQP4表达量较健侧升高。相关性分析显示，将6号大鼠剔除后，1~5号大鼠的AQP ADC值与AQP4表达量呈负相关。结论：AQP MRI能够在体显示AQP4的表达量及分布情况，具有广阔的临床应用价值。

Abstract

Objective: To explore the correlation between aquaporin magnetic resonance molecular imaging(AQP MRI) and the expression of AQP4. Methods: Ten adult SD male rats were subjected to either predisposing(LRP) or sham surgery and then subsequently underwent right middle cerebral artery occlusion(MCAo) for one hour. AQP MRI was executed 48 h after reperfusion. And the AQP4 expression was detected by Western blot. Results: The success rate of modeling was 60%, meaning there were only 6 rats living up to the criteria of modeling. AQP MRI showed AQP ADC values of the affected side of rats No.1~5 were lower than those of the uninjured side, but the AQP ADC values in the affected side of rat No.6 was much higher than that of the uninjured side. Western blot results showed that the AQP4 expression in the affected side of No.1~6 rats was higher than that of the uninjured side. Correlation analysis showed that the AQP ADC values were negatively correlated to the level of AQP4 protein expression when excluding rat No.6. Conclusion: AQP MRI can display the expression quantity as well as the distribution of AQP4 in vivo, and it has great value in clinical application.

导出引用

陈秋雁，吴富淋，彭晓澜，李春丽，江敏，陈婷婷，魏鼎泰. 水通道蛋白磁共振分子成像与水通道蛋白4表达的相关性研究[J]. 中国临床医学影像杂志. 2016, 27(12): 837-841

CHEN Qiu-yan, WU Fu-lin, PENG Xiao-lan, LI Chun-li, JIANG Min, CHEN Ting-ting, WEI Ding-tai. Research on correlation between aquaporin magnetic resonance molecular imaging and AQP4 expression[J]. Journal of China Clinic Medical Imaging. 2016, 27(12): 837-841

中图分类号： Ｒ743 Ｒ445.2

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