目的：探索磁共振弥散成像（MR-DWI）活体检测结肠癌耐药性的可能性。方法：对耐药组与不耐药组人类结肠癌SW480荷瘤裸鼠（各5只）的肿瘤行MR-DWI检查，随后处死荷瘤鼠、切下肿瘤检测肿瘤的PKC、P-gp、MRP1蛋白表达（Western Blot），组织切片观察（HE染色）；对比分析两组荷瘤鼠肿瘤MR-DWI的ADC值及蛋白表达和组织切片改变。结果：不耐药人类结肠癌SW480组裸鼠模型肿瘤边缘及中心ADC值均较耐药人类结肠癌SW480/5-FU组裸鼠模型增高，不耐药人类结肠癌SW480组裸鼠模型肿瘤边缘ADC值与耐药人类结肠癌SW480/5-FU组裸鼠模型肿瘤边缘ADC值的差别具有统计学意义（P＜0.05），肿瘤中心ADC值差别则无统计学意义（P＞0.05）；肿瘤的ADC值与耐药性呈负相关。耐药组PKC、P-gp、MRP1蛋白表达明显高于不耐药组，差别有统计学差异（P＜0.05）。结论：MR-DWI检测的ADC值变化有可能有助于利用MR活体检测结肠癌耐药性。
Abstract
Objective: To investigate the possibilities in detecting the drug resistance of human colon cancer with MR diffusion-weighted imaging(MR-DWI). Methods: SW480(drug response) and SW480/5-FU(5-FU resistance) human colon cancer-bearing nude mice(five each group) were given MR-DWI examinations. Then these mouse models were sacrificed. Their tumors were removed for PKC, P-gp, MRP1 protein expression(Western Blot), and tissue sections(HE staining) was observed. ADCs of the tumor in tumor-bearing mice of two groups were analyzed in comparison with PKC, P-gp, MRP1 protein expression and changes in tissue sections. Results: The values of apparent diffusion coefficient(ADC) detected in the center and edge of the tumor in SW480 group were higher than those in SW480/5-FU group. There was statistical difference(P＜0.05) of ADC value in the edge of tumor between SW480 group and SW480/5-FU group, and there was no statistical difference(P＞0.05) of ADC value in the center of tumor between two groups. ADC value of tumor was negatively correlated with drug resistance. PKC, P-gp, MRP1 protein expression of SW480/5-FU group were significantly higher than that of SW480 group(P<0.05). Conclusions: MR-DWI ADC value change has the potential to help detection of colon cancer drug resistance with MR in vivo.
关键词
结肠肿瘤 /
磁共振成像 /
弥散 /
动物实验
Key words
Colonic neoplasms /
Diffusion magnetic resonance imaging /
Animal Experimentation
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基金
广东省自然科学基金（2015A030313732）。
