目的：探讨多b值扩散加权成像（DWI）无创性评价恩度对人结肠癌裸鼠移植瘤疗效的应用价值。方法：人结肠癌HT-29细胞株接种于48只裸鼠皮下，将48只裸鼠随机分为2组，对照组和恩度组各24只，分别于治疗前及治疗后2 d、7 d、14 d进行多b值扩散加权成像检查，观察表观扩散系数ADC值的变化，分别于治疗后2 d、7 d、14 d扫描后每组随机处死8只裸鼠进行病理免疫组化检查。结果：治疗后恩度组肿瘤ADC10b、ADChigh值逐渐升高，ADClow、ADCperf值逐渐减低，治疗前后不同时间点4组观察指标差异比较有统计学意义（F值分别为29.986、23.742、21.598、63.973，P＜0.05），恩度组组内两两比较，ADC10b、ADChigh、ADCperf治疗前、治疗后2 d、7 d、14 d肿瘤观察指标差异有统计学意义，ADClow除治疗后2 d、7 d组内比较差异无统计学意义，余组内两两比较观察指标差异有统计学意义。恩度组治疗后3个时间点ADCperf与MVD结果呈正相关（P＜0.05），ADC10b与MVD结果呈负相关（P＜0.05）。结论：多b值扩散加权成像扫描可以动态评价人结肠癌裸鼠移植瘤模型在药物治疗后肿瘤组织的变化，可以被认为是早期动态监测抗肿瘤血管生成药物疗效的参考指标。
Abstract
Objective: To discuss the value of multi-b value DWI in evaluating efficacy of endostar in the human colon carcinoma xenograft tumor in nude mice non-invasively. Methods: HT-29 human colon cancer cells were seeded in 48 nude mice. The 48 mice were randomly divided into two groups, the control group and the experimental group, with 24 mice in each. Multi-b value DWI was acquired before, 2 days, 7 days and 14 days after treatment. Change of apparent diffusion coefficient(ADC) was observed. Eight nude mice were sacrificed after scanning 2 days, 7 days and 14 days after treatment, and immunohistochemical examination was done. Results: After treatment, ADC10b and ADChigh values of the tumor in endostar group increased gradually. ADClow and ADCperf values of the tumor in endostar group decreased gradually. There was statistically significant difference among four groups at different time points before and after treatment(F=29.986, 23.742, 21.598, 63.973, P＜0.05). In a paired comparison between time points, ADC10b, ADChigh and ADCperf were statistically different before, 2 days, 7 days and 14 days after treatment. ADClow was statistically different between different time points, but not between 2 days and 7 days after treatment. The other indexes of the tumor were statistically different in the comparison of every two time points. In the experimental group, the ADCperf value was positively correlated with microvessel density(MVD) at three time points(P＜0.05) and the ADC10b value was negatively correlated with MVD(P＜0.05). Conclusion: Multi-b value DWI can evaluate dynamically the change of human colon tumor xenograft model in nude mice after drug treatment, which can be considered as a reference index of early anti-angiogenesis drug efficacy.
关键词
结肠肿瘤；肿瘤移植；小鼠 /
裸；磁共振成像
Key words
Colonic neoplasms /
Neoplasm transplantation /
Mice, nude /
Magnetic resonance imaging
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基金
国家自然科学基金项目（NO.81360214）
