目的：通过超声量化指标观察白藜芦醇（Resveratrol，RES）对CCl4诱导的大鼠肝纤维化模型的影响。方法：20只健康雄性SD大鼠随机分为4组：对照组、RES组、模型组、RES干预组。造模8周后超声检测门静脉近段与远段内径、血流速度及肝组织杨氏模量，大鼠肝组织HE染色，光镜下判定肝纤维化程度。结果：①RES干预后大鼠症状较轻，体质量有所增加，肝纤维化病理得到改善。②大鼠门静脉近段与远段内径在模型组与RES干预组较对照组与RES组均增宽（P<0.05）；门静脉血流速度在模型组与RES干预组较对照组与RES组均增加，但在各组间比较差异均无统计学意义（P>0.05）；肝组织杨氏模量在模型组与RES干预组较对照组与RES组均升高（P<0.05）。RES干预后门静脉内径及杨氏模量改变幅度有所减低。各指标在对照组与RES组间比较差异均无统计学意义（P>0.05）。结论：①RES可以有效改善CCl4诱导的大鼠肝纤维化病理改变。②门静脉内径与杨氏模量可以有效评价RES对CCl4诱导大鼠肝纤维化的影响。
Abstract
Objective: To investigate the effection of resveratrol(RES) on the CCl4-induced liver fibrosis in rat model by ultrasound quantitative index. Methods: Twenty male Sprague-Dawley rats were randomly divided into four groups: control group, RES group, model group, and RES intervention group. The proximal and distal diameter, blood flow velocity of portal vein and liver tissue Young’s modulus were detected by ultrasonography. The degree of hepatic fibrosis was determined under light microscope after HE stained. Results: ①After RES intervention, the symptoms of rats were mild with the weight increase, and the liver fibrosis was improved. ②In model and RES intervention group, the portal vein proximal and distal diameter, Young’s modulus of liver tissue were increased compared with control and RES group(P<0.05); The portal vein blood flow velocity was increased, but the differences among the groups were not statistically significant(P>0.05). The changes of the portal vein diameter and Young’s modulus were decreased after RES intervention. There were no significant differences between the control and RES group(P>0.05). Conclusion: ①RES can effectively improve the pathological changes of CCl4-induced liver fibrosis. ②Portal vein diameter and Young’s modulus can effectively evaluate the effect of RES on CCl4-induced liver fibrosis in rat.
关键词
肝硬化 /
大鼠 /
超声检查
Key words
Liver cirrhosis /
Rats /
Ultrasonography
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参考文献
[1]Snyder JC, Zemke AC, Stripp BR. Reparative capacity of airway epithelium impacts deposition and remodeling of extracellular matrix[J]. Am J Respir Cell Mol Biol, 2009, 40(6): 633-642.
[2]Vilgrain V. Ultrasound of diffuse liver disease and portal hypertension[J]. Eur Radiol, 2001, 11(9): 1563-1577.
[3]Pfeifer L, Zopf S, Siebler J, et al. Prospective Evaluation of Acoustic Radiation Force Impulse(ARFI) Elastography and High-Frequency B-Mode Ultrasound in Compensated Patients for the Diagnosis of Liver Fibrosis/Cirrhosis in Comparison to Mini-Laparoscopic Biopsy[J]. Ultraschall Med, 2015, 36(6): 581-589.
[4]Lee ES, Shin MO, Yoon S, et al. Resveratrol inhibits dimethylnitrosamine-induced hepatic fibrosis in rats[J]. Arch Pharm Res, 2010, 33(6): 925-932.
[5]中华医学会传染病与寄生虫病学分会，肝病学分会. 病毒性肝炎防治方案[J]. 中华肝脏病杂志，2000，8（6）：324-329.
[6]Gebo KA, Herlong HF, Torbenson MS, et al. Role of liver biopsy in management of chronic hepatitis C: a systematic review[J]. Hepatology, 2002, 36(5 Suppl 1): S161-S172.
[7]Kobyliak N, Abenavoli L. The role of liver biopsy to assess non-alcoholic fatty liver disease[J]. Rev Recent Clin Trials, 2014, 9(3): 159-169.
[8]温小凤，蒋忠胜，柯柳，等. 门静脉和肝中静脉内径与肝纤维化相关性的临床研究[J]. 临床超声医学杂志，2008，10（10）：673-675.
[9]陈永鹏，戴琳，冯筱榕，等. 超声波检查对肝脏纤维化分期的诊断价值[J]. 中华肝脏病杂志，2002，10（2）：132-134.
[10]Ferraioli G, Tinelli C, Zicchetti M, et al. Reproducibility of real-time shear wave elastography in the evaluation of liver elasticity[J]. Eur J Radiol, 2012, 81(11): 3102-3106.
[11]Bercoff J, Tanter M, Fink M. Supersonic shear imaging: a new technique for soft tissue elasticity mapping[J]. IEEE Trans Ultrason Ferroelectr Freq Control, 2004, 51(4): 396-409.
[12]Chang JM, Moon WK, Cho N, et al. Clinical application of shear wave elastography(SWE) in the diagnosis of benign and malignant breast diseases[J]. Breast Cancer Res Treat, 2011, 129(1): 89-97.
[13]Ferraioli G, Tinelli C, Dal Bello B, et al. Accuracy of real-time shear wave elastography for assessing liver fibrosis in chronic hepatitis C: a pilot study[J]. Hepatology, 2012, 56(6): 2125-2133.
[14]曾婕，吴莉莉，郑荣琴，等. 实时剪切波弹性成像检测肝脏弹性模量与肝纤维化分期的相关性研究[J]. 中华医学超声杂志：电子版，2012，9（9）：781-784.
[15]Aubé C, Winkfield B, Oberti F, et al. New Doppler ultrasound signs improve the non-invasive diagnosis of cirrhosis or severe liver fibrosis[J]. Eur J Gastroenterol Hepatol, 2004, 16(8): 743-751.
[16]Lutz HH, Schroeter B, Kroy DC, et al. Doppler Ultrasound and Transient Elastography in Liver Transplant Patients for Noninvasive Evaluation of Liver Fibrosis in Comparison with Histology: A Prospective Observational Study[J]. Dig Dis Sci, 2015, 60(9): 2825-2831.
[17]Hirata M, Akbar SM, Horiike N, et al. Noninvasive diagnosis of the degree of hepatic fibrosis using ultrasonography in patients with chronic liver disease due to hepatitis C virus[J]. Eur J Clin Invest, 2001, 31(6): 528-535.
[18]Bernatik T, Strobel D, Hahn EG, et al. Doppler measurements: a surrogate marker of liver fibrosis?[J]. Eur J Gastroenterol Hepatol, 2002, 14(4): 383-387.
