目的：通过1H-MRS成像在体检测新生猪缺氧缺血（Hypoxic ischemia，HI）脑损伤模型在损伤后不同时间点内Tau蛋白及谷氨酸（Glu）含量的变化趋势，进一步明确Tau蛋白及Glu在HI后脑损伤中的相互作用机制。方法：选用新生约克夏猪45只，3~5 d日龄，体质量1~1.5 kg，性别雄性（为尽量减少性别带来的个体差异）。对照组9头，HI模型组36头。通过1H-MRS成像在体检测HI后不同时间点基底节区Tau蛋白及Glu含量的变化，统计Tau蛋白及Glu在各时间点间的统计学差异，以及HI后Tau蛋白与Glu随时间变化的相关性，P<0.05为有统计学差异，小数保留2位。结果：1H-MRS结果显示，HI后Tau蛋白含量呈现先升高随后稍降低的趋势，在24~48 h达到峰值，与对照组具有统计学差异（P<0.05）；Glu含量呈先上升后降低的趋势，继而又上升，即“双峰”样改变，在6~12 h、24~48 h达到峰值。Tau蛋白及Glu变化呈正相关，相关系数r=0.76（P<0.05）。结论：HI后，Tau蛋白及Glu变化呈正相关，二者在HI后共同调节脑内神经网络、神经元及胶质细胞。
Abstract
Objective: To investigate the Tau protein expression and glutamate(Glu) characteristics in basal ganglia following hypoxic-ischemic(HI) reperfusion brain injury in a piglet model, further defined the Tau protein and Glu in the role of HI brain injury mechanism. Methods: A total of 45 healthy piglets(3~5 days old, 1.0~1.5 kg) were divided into control(n=9) and hypoxic-ischemic(HI) model groups(n=36). The HI model group was further divided into six groups according to 1H-magnetic resonance spectroscopy(1H-MRS) scan times after HI(0~2 h, 2~6 h, 6~12 h, 12~24 h, 24~48 h and 48~72 h). Measure Tau protein and Glu at different time points after HI in basal ganglia and the correlation of Tau protein and Glu, P<0.05 considered statistically significant, the decimal to 2 digits. Results: The results of 1H-MRS showed that after HI, the Tau protein content increased first, then decreased, and reached the highest point in 24~48 h, which was statistically different from the control group. Glu appears to rise first, then decreases, then rises again, showing a “bimodal” change, which peaks at 6~12 h and 24~48 h. Tau protein and Glu were positively correlated, correlation coefficient r=0.76(P<0.05). Conclusions: These results indicate that after HI, Tau protein and Glu work together on the neural networks and neurons and glial cells in the brain.
关键词
脑损伤 /
tau蛋白质类 /
谷氨酸 /
猪 /
雏型 /
磁共振成像
Key words
Brain injuries /
tau proteins /
Glutamic acid /
Swine, miniature /
Magnetic resonance imaging
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基金
国家自然科学基金（No.30570541，30770632，81271631）；盛京医院自由研究者基金（No.2014-02）；辽宁省临床能力建设项目（LNCCC-B06-2014）。
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