大鼠C6脑胶质瘤的CT灌注成像研究

关丽明; 徐克; 郭敏; 戚喜勋; 方长兴; 刘树立

中国临床医学影像杂志 ›› 2007, Vol. 18 ›› Issue (12) : 846-850.

论著

大鼠C6脑胶质瘤的CT灌注成像研究

关丽明，徐克，郭敏，戚喜勋，方长兴，刘树立

作者信息 +

CT perfusion C6 cerebral gliomas in rat

GUAN Li-ming, XU Ke, GUO Min, QI Xi-xun, FANG Chang-xing, LIU Shu-li

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文章历史 +

摘要

目的：运用CT灌注成像评价大鼠C6脑胶质瘤的生长及其血管生成。方法：采用立体定向方法在30只雌性Wistar大鼠右侧尾状核接种C6细胞，于接种后1w、2w、3w分别进行CT灌注成像，每次检查结束后留取鼠脑标本作病理学检查。结果：荷瘤1w组肿瘤区各灌注参数值均比对侧正常脑组织增大（P<0.05），但没有明显新生肿瘤血管；荷瘤2w及3w组肿瘤区CBF，BV，PS值和瘤脑交界处PS值均比对侧正常脑组织明显增大，肿瘤中心、肿瘤周边和瘤脑交界处之间仅PS值差别具有统计学意义，肿瘤区微血管密度（MVD）值明显增大，以肿瘤周边更为明显，其差别具有统计学意义（P<0.05）；整个观察期内肿瘤中心和周边区域CBV值及肿瘤中心PS值与相应的MVD值呈等级正相关，rs分别为0.576、0.482和0.646（P<0.05）。结论：CT灌注成像可以反映大鼠C6脑胶质瘤生长过程中肿瘤区域不同部位微循环的动态变化，其中肿瘤周边区域CBV和PS值变化明显，反映了血管生成。

Abstract

Objective: To evaluate growth and angiogenesis of C6 cerebral gliomas in rat by CT. Methods: C6 cells were inoculated into the right caudate nucleus of 30 Wistar rats by stereotactic procedure to established animal model. CT perfusion was performed at 1 week, 2 and 3 weeks after implantation. Immediately after each examination, some rats were sacrificed for pathology. Results: Although there was no angiogenesis found, all the values of perfusion parameters for the tumor at 1 week after implantation increased compared to the contralateral side(P<0.05). The values of CBF, CBV, PS in the tumor and PS in the tumor-brain interface at 2 and 3 weeks after implantation were higher than that in the regions of contralateral brain, only the PS value had statistical difference in comparing the center of the tumor with the periphery of the tumor and the tumor-brain interface. The microvascular density(MVD) within the tumor, especially at the periphery of the tumor, increased significantly compared to the contralateral side(P<0.05). The perfusion volume and permeability increased with growth of the tumor during 3 weeks after implantation, in which positive correlation was present not only between CBV and MVD in the center and at the periphery of the tumor but also PS and MVD in the center of the tumor(rs=0.576, 0.482, 0.646 respectively, P<0.05). Conclusion: The parameters of CT perfusion can evaluate tumor microcirculation with the development of rat C6 brain gliomas in vivo, while CBV and PS, change obviously at the periphery of the tumor, can be the more sensitive index reflecting tumoral angiogenesis.

导出引用

关丽明;徐克;郭敏;戚喜勋;方长兴;刘树立. 大鼠C6脑胶质瘤的CT灌注成像研究[J]. 中国临床医学影像杂志. 2007, 18(12): 846-850

GUAN Li-ming;XU Ke;GUO Min;QI Xi-xun;FANG Chang-xing;LIU Shu-li. CT perfusion C6 cerebral gliomas in rat[J]. Journal of China Clinic Medical Imaging. 2007, 18(12): 846-850

中图分类号： Ｒ739.41 Ｒ814.42