摘要
目的：探讨99mTc-MIBI门控静息心肌显像在评价转染携带人肝细胞生长因子的腺病毒（Ad5-HGF）治疗心肌梗死的价值。方法：25只小型猪随机分成对照组、模型组和3个治疗组（Ad5-HGF低、中、高剂量组），均在手术前及观察期结束时分别接受门控心肌显像和冠状动脉造影，并行组织学检查。结果：①治疗前模型组及各治疗组心肌灌注和Rentrop计分均明显低于对照组（P<0.01）；低剂量组治疗前、后心肌灌注和Rentrop计分差值与模型组比较没有明显差别（P＞0.05）；中、高剂量组治疗前、后差值明显高于模型组（P<0.01）。②治疗前模型组及各治疗组左室射血分数均明显低于对照组（P<0.01）；低剂量组治疗前、后左室射血分数差值与模型组比较没有明显差别（P＞0.05）；中、高剂量组治疗前、后差值明显高于模型组（P<0.05）。③模型组血管数量和低剂量组比较没有差别（P＞0.05），但明显少于对照组和中、大剂量组（P<0.01）。结论：可以利用99mTc-MIBI门控静息心肌显像来评价Ad5-HGF治疗猪心肌梗死的疗效。
Abstract
Objective: To study the value of rest 99mTc-MIBI gated myocardial perfusion imaging(G-MPI) in evaluating the curative effects of adenovirus-mediated human hepatocyte growth factor(Ad5-HGF) transference in the swine model of myocardial infarction. Methods: Twenty-five swine were randomly divided into 5 groups: control group, model group and Ad5-HGF-treated group(low, medium and high dose). G-MPI and coronary angiography(CAG) were performed before and after treatment, respectively. Histological examination was also performed at the end of the study. Results: ①The myocardial perfusion and Rentrop scores of the model group and all treated groups were significantly lower than that of the control group before treatment(P<0.01). The score difference before and after treatment between the low dose group and the model group was not statistically significant(P>0.05), whereas the score difference between the medium and high dose group was significantly higher than the model group(P<0.01). ②Left ventricular ejection fraction(LVEF) measured by G-MPI of the model group and all treated groups were significantly lower than that of the control group before treatment(P<0.01). The LVEF difference before and after treatment between the low dose group and the model group was not significant(P>0.05), whereas the LVEF difference of the medium and high dose group was significantly higher than the model group(P<0.01). ③In the medium and high dose group, the number of vessels were significantly more than that of the model group(P<0.01). Conclusion: G-MPI is an excellent tool to evaluate the curative effects of Ad5-HGF transference in the swine model of myocardial infarction.
关键词
心肌梗塞 /
猪 /
99m锝甲氧基异丁基异腈
Key words
myocardial infarction /
swine /
technetium tc 99m sestamibi
冯建林;李殿富;程 旭;徐兆强;张友荣;李建华;袁冬兰.
门控心肌显像评价肝细胞生长因子转染小型猪心肌梗死的实验研究[J]. 中国临床医学影像杂志. 2007, 18(4): 247-250
FENG Jian-lin;LI Dian-fu;CHENG Xu;XU Zhao-qiang;ZHANG You-rong;LI Jian-hua;YUAN Dong-lan.
Value of gated myocardial perfusion imaging in evaluating curative effectsof Ad5-HGF transference in swine model of myocardial infarction[J]. Journal of China Clinic Medical Imaging. 2007, 18(4): 247-250
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